TY - JOUR
T1 - MiR-27b-regulated TCTP as a novel plasma biomarker for oral cancer
T2 - From quantitative proteomics to post-transcriptional study
AU - Lo, Wan Yu
AU - Wang, Huang Joe
AU - Chiu, Chih Wei
AU - Chen, Sung Fang
N1 - Funding Information:
This study was supported by grants from the National Science Council of Taiwan ( NSC 96-2320-B-039-024 , NSC 97-2320-B-039-016-MY3 , NSC 98-2113-M-003-007-MY2 , and NSC 100-2113-M-003-002-MY2 ), the China Medical University Hospital ( DMR-98-100 ) and China Medical University ( CMU100-S-04 ).
PY - 2012
Y1 - 2012
N2 - We combined an iTRAQ-based quantitative proteomic analysis and the miRNA determination to profile potentially novel biomarker from oral cancer. There are 757 and 674 unique proteins identified from proteomic analysis, and 13 proteins displayed consistent underexpression (< 0.67 fold) in normal tissues in comparison with the corresponding tumor tissues. After preliminary screening, EGFR, OAT, TPT1, ITGA6, G3BP1 and CB39L were the six genes validated in the 37 oral cancer patients (T1, n = 10; T2, n = 10; T3, n = 10 and T4, n = 7). The TPT1, ITGA6 and CAB39L genes were displayed the higher transcriptions level in the tumor tissues and the TPT1, ITGA6 and CAB39L proteins were also shown overexpression in the tumor tissues from the same patients. The miR-19a, 19b, 27a, 27b, 186, 203 and 377 transcripts were predicted and the miR-27b level was shown to significantly reduce in the tumor tissues and the plasma of OSCC patients. In the in vitro study, the overexpression of miR-27b only significantly decreased TCTP protein and gene levels in both HSC-3 and Cal-27 cell lines. Our results demonstrate that human miR-27b regulates the expression of the TCTP tumor protein, and circulating miR-27b may be useful as a biomarker for oral cancer research.
AB - We combined an iTRAQ-based quantitative proteomic analysis and the miRNA determination to profile potentially novel biomarker from oral cancer. There are 757 and 674 unique proteins identified from proteomic analysis, and 13 proteins displayed consistent underexpression (< 0.67 fold) in normal tissues in comparison with the corresponding tumor tissues. After preliminary screening, EGFR, OAT, TPT1, ITGA6, G3BP1 and CB39L were the six genes validated in the 37 oral cancer patients (T1, n = 10; T2, n = 10; T3, n = 10 and T4, n = 7). The TPT1, ITGA6 and CAB39L genes were displayed the higher transcriptions level in the tumor tissues and the TPT1, ITGA6 and CAB39L proteins were also shown overexpression in the tumor tissues from the same patients. The miR-19a, 19b, 27a, 27b, 186, 203 and 377 transcripts were predicted and the miR-27b level was shown to significantly reduce in the tumor tissues and the plasma of OSCC patients. In the in vitro study, the overexpression of miR-27b only significantly decreased TCTP protein and gene levels in both HSC-3 and Cal-27 cell lines. Our results demonstrate that human miR-27b regulates the expression of the TCTP tumor protein, and circulating miR-27b may be useful as a biomarker for oral cancer research.
KW - ITRAQ
KW - MiR-27b
KW - Oral cancer
KW - Quantitative proteomic analysis
KW - TCTP
KW - Translationally controlled tumor protein
UR - http://www.scopus.com/inward/record.url?scp=84870394042&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870394042&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.07.039
DO - 10.1016/j.jprot.2012.07.039
M3 - Article
C2 - 22902387
AN - SCOPUS:84870394042
SN - 1874-3919
VL - 77
SP - 154
EP - 166
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -
