Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

Yu Cheng Hsu, Shou-Hsien Li, Yao Sung Lin, Lucia Liu Severinghaus

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

Original languageEnglish
Pages (from-to)161-165
Number of pages5
JournalConservation Genetics
Volume7
Issue number1
DOIs
Publication statusPublished - 2006 Feb 1

Fingerprint

Strigidae
Strigiformes
Otus
Microsatellite Repeats
microsatellite repeats
loci
genetic marker
reproductive strategy
population genetics
polymorphism
phylogenetics
Population Genetics
Genetic Markers
mating systems
Libraries
genetic polymorphism
genetic markers
phylogeny

Keywords

  • DNA polymorphism
  • Genotyping
  • Microsatellite
  • Microsatellites
  • Otus elegans
  • Strigidae

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae. / Hsu, Yu Cheng; Li, Shou-Hsien; Lin, Yao Sung; Severinghaus, Lucia Liu.

In: Conservation Genetics, Vol. 7, No. 1, 01.02.2006, p. 161-165.

Research output: Contribution to journalArticle

@article{0732cd7208cf43518a9232232a2fa9be,
title = "Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae",
abstract = "We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100{\%}) were polymorphic in the three Otus owls but only five (27.8{\%}) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.",
keywords = "DNA polymorphism, Genotyping, Microsatellite, Microsatellites, Otus elegans, Strigidae",
author = "Hsu, {Yu Cheng} and Shou-Hsien Li and Lin, {Yao Sung} and Severinghaus, {Lucia Liu}",
year = "2006",
month = "2",
day = "1",
doi = "10.1007/s10592-005-5477-x",
language = "English",
volume = "7",
pages = "161--165",
journal = "Conservation Genetics",
issn = "1566-0621",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

AU - Hsu, Yu Cheng

AU - Li, Shou-Hsien

AU - Lin, Yao Sung

AU - Severinghaus, Lucia Liu

PY - 2006/2/1

Y1 - 2006/2/1

N2 - We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

AB - We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

KW - DNA polymorphism

KW - Genotyping

KW - Microsatellite

KW - Microsatellites

KW - Otus elegans

KW - Strigidae

UR - http://www.scopus.com/inward/record.url?scp=33644525878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644525878&partnerID=8YFLogxK

U2 - 10.1007/s10592-005-5477-x

DO - 10.1007/s10592-005-5477-x

M3 - Article

AN - SCOPUS:33644525878

VL - 7

SP - 161

EP - 165

JO - Conservation Genetics

JF - Conservation Genetics

SN - 1566-0621

IS - 1

ER -