Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae

Yu Cheng Hsu, Shou Hsien Li, Yao Sung Lin, Lucia Liu Severinghaus

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.

Original languageEnglish
Pages (from-to)161-165
Number of pages5
JournalConservation Genetics
Volume7
Issue number1
DOIs
Publication statusPublished - 2006 Feb 1

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Keywords

  • DNA polymorphism
  • Genotyping
  • Microsatellite
  • Microsatellites
  • Otus elegans
  • Strigidae

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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