Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

Hsuan Ming Huang; Cheng Huang Lin

doi:10.1016/j.jchromb.2004.11.018

Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

Hsuan Ming Huang, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 ⁵ and ∼2 × 10⁵ by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 10⁶ when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10^-7 and 1.2 × 10^-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10^-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

Original language	English
Pages (from-to)	113-119
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	816
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2004.11.018
Publication status	Published - 2005 Feb 25

Keywords

Capillary electrophoresis
Dopamine
Methanol plug
Sweeping-MEKC
Violet light emitting diode

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/j.jchromb.2004.11.018

Cite this

Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection. / Huang, Hsuan Ming; Lin, Cheng Huang.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 816, No. 1-2, 25.02.2005, p. 113-119.

Research output: Contribution to journal › Article › peer-review

Huang, HM & Lin, CH 2005, 'Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 816, no. 1-2, pp. 113-119. https://doi.org/10.1016/j.jchromb.2004.11.018

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title = "Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection",

abstract = "The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.",

keywords = "Capillary electrophoresis, Dopamine, Methanol plug, Sweeping-MEKC, Violet light emitting diode",

author = "Huang, {Hsuan Ming} and Lin, {Cheng Huang}",

note = "Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-92-2113-M-003-023.",

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AU - Lin, Cheng Huang

N1 - Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-92-2113-M-003-023.

PY - 2005/2/25

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N2 - The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

AB - The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

KW - Capillary electrophoresis

KW - Dopamine

KW - Methanol plug

KW - Sweeping-MEKC

KW - Violet light emitting diode

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Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

Abstract

Keywords

ASJC Scopus subject areas

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