Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection

Hsuan Ming Huang, Cheng Huang Lin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ∼1 × 10 5 and ∼2 × 105 by means of MEKC and sweeping-MEKC and this was improved to ∼1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume816
Issue number1-2
DOIs
Publication statusPublished - 2005 Feb 25

Keywords

  • Capillary electrophoresis
  • Dopamine
  • Methanol plug
  • Sweeping-MEKC
  • Violet light emitting diode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection'. Together they form a unique fingerprint.

  • Cite this