Mass production and dynamic imaging of fluorescent nanodiamonds

Yi Ren Chang, Hsu Yang Lee, Kowa Chen, Chun Chieh Chang, Dung Sheng Tsai, Chi Cheng Fu, Tsong Shin Lim, Yan Kai Tzeng, Chia Yi Fang, Chau Chung Han, Huan Cheng Chang*, Wunshain Fann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

725 Citations (Scopus)


Fluorescent nanodiamond is a new nanomaterial that possesses several useful properties, including good biocompatibility, excellent photostability and facile surface functionalizability. Moreover, when excited by a laser, defect centres within the nanodiamond emit photons that are capable of penetrating tissue, making them well suited for biological imaging applications. Here, we show that bright fluorescent nanodiamonds can be produced in large quantities by irradiating synthetic diamond nanocrystallites with helium ions. The fluorescence is sufficiently bright and stable to allow three-dimensional tracking of a single particle within the cell by means of either one- or two-photon-excited fluorescence microscopy. The excellent photophysical characteristics are maintained for particles as small as 25 nm, suggesting that fluorescent nanodiamond is an ideal probe for long-term tracking and imaging in vivo, with good temporal and spatial resolution.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalNature Nanotechnology
Issue number5
Publication statusPublished - 2008 May
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • General Materials Science
  • Condensed Matter Physics
  • Electrical and Electronic Engineering


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