Magnetically enhanced high-specificity virus detection using bio-activated magnetic nanoparticles with antibodies as labeling markers

S. Y. Yang, W. C. Wang, C. B. Lan, C. H. Chen, J. J. Chieh, H. E. Horng, Chin Yih Hong, H. C. Yang, C. P. Tsai, C. Y. Yang, I. C. Cheng, W. C. Chung

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    This study describes magnetically driven suppression of cross-reactions among molecules. First, the magnetic nanoparticles are coated with bio-probes and dispersed in liquid. The bio-probes can then bind with homologous or heterologous bio-targets. When alternating-current (ac) magnetic fields are applied, magnetic nanoparticles rotate driven by ac magnetic fields. Thus, the bio-targets bound on the surface of magnetic nanoparticles experience a centrifugal force. The centrifugal force can be manipulated by adjusting the angular frequency of the rotating magnetic nanoparticles. The angular frequency is determined by the applied ac magnetic field frequency. Since the binding force for good binding is much higher than that of poor binding, frequency manipulation is needed for the centrifugal force to be higher than the poor-binding force but lower than the good-binding force. Therefore, poor binding which contributes to cross reactions between molecules can be suppressed efficiently by control of the ac magnetic field frequency.

    Original languageEnglish
    Pages (from-to)14-18
    Number of pages5
    JournalJournal of Virological Methods
    Volume164
    Issue number1-2
    DOIs
    Publication statusPublished - 2010 Mar 1

    Keywords

    • High specificity
    • Magnetic field
    • Magnetic nanoparticles
    • Virus

    ASJC Scopus subject areas

    • Virology

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