Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, N. S. Hing, H. E. Horng, Chin Yih Hong, H. C. Yang, C. F. Chang, H. Y. Lin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection.

Original languageEnglish
Pages (from-to)681-685
Number of pages5
JournalJournal of Magnetism and Magnetic Materials
Volume323
Issue number6
DOIs
Publication statusPublished - 2011 Mar 1

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Keywords

  • DNA
  • Immunomagnetic reduction
  • Magnetic nanoparticle

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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