Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

S. Y. Yang, J. J. Chieh, W. C. Wang, C. Y. Yu, N. S. Hing, H. E. Horng, Chin Yih Hong, H. C. Yang, C. F. Chang, H. Y. Lin

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection.

    Original languageEnglish
    Pages (from-to)681-685
    Number of pages5
    JournalJournal of Magnetism and Magnetic Materials
    Volume323
    Issue number6
    DOIs
    Publication statusPublished - 2011 Mar

    Keywords

    • DNA
    • Immunomagnetic reduction
    • Magnetic nanoparticle

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics

    Fingerprint Dive into the research topics of 'Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay'. Together they form a unique fingerprint.

    Cite this