Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay

Chin Yih Hong, Shieh Yueh Yang, Herng Er Horng, Hong Chang Yang

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    A method involving the use of magnetic nanoparticles to suppress the cross-reactions in immunoassay is developed. Antibodies are coated onto magnetic nanoparticles. These antibodies bind with target and non-target molecules. Once an alternative-current magnetic field is applied, magnetic nanoparticles oscillate with the magnetic field. The target and non-target molecules attached onto magnetic nanoparticles via antibodies experience a centrifugal force, which is against the association between antibodies and target/non-target molecules. Theoretically, the centrifugal force is proportional to the square of the frequency of the applied magnetic field. Thus, the strength of the centrifugal force can be manipulated by changing the frequency of the applied magnetic field. By well controlling the frequency of applied magnetic field, the centrifugal force can be stronger than the binding between antibodies and non-target molecules, but still weaker than that of target molecules. Consequently, the binding between antibodies and non-target molecules is broken by the centrifugal force.

    Original languageEnglish
    Title of host publicationASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010
    Pages393-396
    Number of pages4
    Volume4
    DOIs
    Publication statusPublished - 2010 Dec 1
    EventASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010 - Montreal, QC, Canada
    Duration: 2010 Aug 152010 Aug 18

    Other

    OtherASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010
    CountryCanada
    CityMontreal, QC
    Period10/8/1510/8/18

    Fingerprint

    Immunoassay
    Biomolecules
    Centrifugal Force
    Antibody
    Antibodies
    Magnetic Nanoparticles
    Molecules
    Magnetic Field
    Magnetic fields
    Nanoparticles
    Target
    Directly proportional
    Alternatives

    ASJC Scopus subject areas

    • Modelling and Simulation
    • Mechanical Engineering
    • Computer Science Applications
    • Computer Graphics and Computer-Aided Design

    Cite this

    Hong, C. Y., Yang, S. Y., Horng, H. E., & Yang, H. C. (2010). Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay. In ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010 (Vol. 4, pp. 393-396) https://doi.org/10.1115/DETC2010-28040

    Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay. / Hong, Chin Yih; Yang, Shieh Yueh; Horng, Herng Er; Yang, Hong Chang.

    ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010. Vol. 4 2010. p. 393-396.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Hong, CY, Yang, SY, Horng, HE & Yang, HC 2010, Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay. in ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010. vol. 4, pp. 393-396, ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010, Montreal, QC, Canada, 10/8/15. https://doi.org/10.1115/DETC2010-28040
    Hong CY, Yang SY, Horng HE, Yang HC. Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay. In ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010. Vol. 4. 2010. p. 393-396 https://doi.org/10.1115/DETC2010-28040
    Hong, Chin Yih ; Yang, Shieh Yueh ; Horng, Herng Er ; Yang, Hong Chang. / Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay. ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE2010. Vol. 4 2010. pp. 393-396
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    abstract = "A method involving the use of magnetic nanoparticles to suppress the cross-reactions in immunoassay is developed. Antibodies are coated onto magnetic nanoparticles. These antibodies bind with target and non-target molecules. Once an alternative-current magnetic field is applied, magnetic nanoparticles oscillate with the magnetic field. The target and non-target molecules attached onto magnetic nanoparticles via antibodies experience a centrifugal force, which is against the association between antibodies and target/non-target molecules. Theoretically, the centrifugal force is proportional to the square of the frequency of the applied magnetic field. Thus, the strength of the centrifugal force can be manipulated by changing the frequency of the applied magnetic field. By well controlling the frequency of applied magnetic field, the centrifugal force can be stronger than the binding between antibodies and non-target molecules, but still weaker than that of target molecules. Consequently, the binding between antibodies and non-target molecules is broken by the centrifugal force.",
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