Lunasin attenuates obesity-related inflammation in RAW264.7 cells and 3T3-L1 adipocytes by inhibiting inflammatory cytokine production

Chia Chien Hsieh, Mei Jia Chou, Chih Hsuan Wang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Obesity has become a major threat to public health and is accompanied by chronic lowgrade inflammation, which leads to various pathological developments. Lunasin, a natural seed peptide, exhibits several biological activities, such as anti-carcinogenesis, anti-inflammatory, and antioxidant activities. However, the mechanism of action of lunasin in obesityrelated inflammation has not been investigated. The aim of this study was to explore whether lunasin could reduce the inflammation induced by obesity-related mediators in RAW264.7 cells and 3T3-L1 adipocytes and whether it could attenuate the crosstalk between the two cell lines. RAW264.7 cells were cultured in leptin-containing medium, adipocyte- conditioned medium (Ad-CM), or co-cultured with 3T3-L1 cells to mimic the physiology of obesity. The data showed that the secretion of pro-inflammatory cytokine interleukin- 1β (IL-1β) was inhibited by lunasin after leptin activation of RAW264.7 cells. In addition, lunasin decreased monocyte chemoattractant protein-1 (MCP-1) and IL-1β secretions in the Ad-CM model. Cytokine MCP-1, IL-6, tumor necrosis factor (TNF)-α, and IL-1β secretions were significantly decreased by leptin or Ad-CM plus lipopolysaccharide stimulation. Subsequently, the co-culture of the two cells refined the direct relation between them, resulting in apparently increased MCP-1, and decreased IL-6 levels after lunasin treatment. In 3T3-L1 adipocytes, lunasin also exhibited anti-inflammatory property by inhibiting MCP-1, plasminogen activator inhibitor-1, and leptin productions stimulated by (TNF)-α,lipopolysaccharide,or RAW264.7 cell-conditioned medium. This result revealed that lunasin acts as a potential anti-inflammatory agent not only in macrophages but also in adipocytes, disrupting the crosstalk between these two cells. Therefore, this study suggests the intake of lunasin from diet or as a supplement, for auxiliary prevention or therapy in obesity-related inflammatory applications.

Original languageEnglish
Article numbere0171969
JournalPLoS ONE
Volume12
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

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3T3-L1 Cells
Chemokine CCL2
Conditioned Culture Medium
Leptin
adipocytes
Adipocytes
obesity
cytokines
Obesity
inflammation
Interleukin-1
Cytokines
Inflammation
Anti-Inflammatory Agents
leptin
Crosstalk
interleukin-1
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lunasin attenuates obesity-related inflammation in RAW264.7 cells and 3T3-L1 adipocytes by inhibiting inflammatory cytokine production. / Hsieh, Chia Chien; Chou, Mei Jia; Wang, Chih Hsuan.

In: PLoS ONE, Vol. 12, No. 2, e0171969, 01.02.2017.

Research output: Contribution to journalArticle

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