Lunasin attenuates obesity-associated metastasis of 4T1 breast cancer cell through anti-inflammatory property

Chia Chien Hsieh, Chih Hsuan Wang, Yu Shan Huang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Obesity prevalence is increasing worldwide and is accompanied by low-grade inflammation with macrophage infiltration, which is linked with a poorer breast cancer prognosis. Lunasin is a natural seed peptide with chemopreventive properties and multiple bioactivities. This is the first study to explore the chemopreventive effects of lunasin in the obesity-related breast cancer condition using 4T1 breast cancer cells, 3T3-L1 adipocytes, and conditioned media. An obesity-related 4T1 cell proliferation and metastasis. Lunasin treatment inhibited metastasis of breast cancer 4T1 cell proliferation and metastasis. Lunasin treatment inhibited metastasis of breast cancer cells, partially through modestly inhibiting production of the angiogenesis-mediator vascular endothelial growth factor (VEGF) and significantly by inhibiting secretion in the Ad-CM condition. Subsequently, two adipocytes inflammation models, 3T3-L1 adipocytes were stimulated by tumor necrosis factor (TNF)-α, and RAW 264.7 cell-conditioned medium (RAW-CM) was used to mimic the obese microenvironment. Lunasin significantly inhibited interleukin (IL)-6 and macrophage chemoattractant protein (MCP)-1 secretion by TNF-α stimulation, and MCP-1 secretion in the RAW-CM model. This study highlights that lunasin suppressed 3T3-L1 adipocyte inflammation and inhibited 4T1 breast cancer cell migration. Interestingly, lunasin exerted more effective anti-metastasis activity in the obesity-related condition models, indicating that it possesses anti-inflammatory properties and blocks adipocyte-cancer cell cross-talk.

Original languageEnglish
Article number2109
JournalInternational journal of molecular sciences
Volume17
Issue number12
DOIs
Publication statusPublished - 2016 Dec 15

Fingerprint

obesity
metastasis
breast
Macrophages
Adipocytes
Anti-Inflammatory Agents
Conditioned Culture Medium
Obesity
cancer
Cells
Breast Neoplasms
Neoplasm Metastasis
Chemotactic Factors
Cell proliferation
secretions
macrophages
Tumor Necrosis Factor-alpha
Inflammation
Proteins
necrosis

Keywords

  • Adipocyte
  • Breast cancer
  • Inflammation
  • Lunasin
  • Metastasis

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Lunasin attenuates obesity-associated metastasis of 4T1 breast cancer cell through anti-inflammatory property. / Hsieh, Chia Chien; Wang, Chih Hsuan; Huang, Yu Shan.

In: International journal of molecular sciences, Vol. 17, No. 12, 2109, 15.12.2016.

Research output: Contribution to journalArticle

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