Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA2

C. W. Sun, L. J. Chen, L. C. Lin, H. M. Li

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Chloroplasts are a major destination of protein traffic within leaf cells. Protein import into chloroplasts is mediated by a set of translocon complexes at the chloroplast envelope. Current data indicate that the expression of translocon genes is regulated in a tissue-specific manner, possibly to accommodate the higher import demand of chloroplasts in leaves and the lower demand of plastids in other tissues. We have designed a transgene-based positive screen to isolate mutants disrupted in protein import into plastids. The first locus we isolated, CIA2, encodes a protein containing a motif conserved within the CCT family of transcription factors. Biochemical analysis indicates that CIA2 is responsible for specific upregulation of the translocon genes atToc33 and atToc75 in leaves. Identification of CIA2 provides new insights into the tissue-specific regulation of translocon gene expression.

Original languageEnglish
Pages (from-to)2053-2061
Number of pages9
JournalPlant Cell
Volume13
Issue number9
DOIs
Publication statusPublished - 2001 Oct 14

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Chloroplast Genes
Amino Acid Motifs
Chloroplasts
Up-Regulation
chloroplasts
imports
Plastids
plastids
leaves
Proteins
genes
proteins
gene expression regulation
Gene Expression Regulation
Transgenes
traffic
transgenes
Transcription Factors
transcription factors
Gene Expression

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA2. / Sun, C. W.; Chen, L. J.; Lin, L. C.; Li, H. M.

In: Plant Cell, Vol. 13, No. 9, 14.10.2001, p. 2053-2061.

Research output: Contribution to journalArticle

Sun, C. W. ; Chen, L. J. ; Lin, L. C. ; Li, H. M. / Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA2. In: Plant Cell. 2001 ; Vol. 13, No. 9. pp. 2053-2061.
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