TY - JOUR
T1 - Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan
T2 - Fluorescence observation of structure and cytocompatibility
AU - Lee, Yen Hsien
AU - Chang, Jung Jhih
AU - Lai, Wen Fu
AU - Yang, Ming Chien
AU - Chien, Chiang Ting
N1 - Funding Information:
The authors would like to thank the National Science Council of Taiwan, Republic of China , for financially supporting this research under grants NSC-98-2221-E-011-005-MY2, NSC-98-2320-B-002-043-MY3, and NSC-99-2120-M-038-001 . The authors would also like to thank National Taiwan University Hospital for providing grants NTUH 99-S1391 and NTUH 100-S1661 .
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.
AB - In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.
KW - Alginate
KW - Chitosan
KW - Fluorescence labeling
KW - Hydrogel
KW - Poly(γ-glutamic acid)
UR - http://www.scopus.com/inward/record.url?scp=79957666012&partnerID=8YFLogxK
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U2 - 10.1016/j.colsurfb.2011.04.002
DO - 10.1016/j.colsurfb.2011.04.002
M3 - Article
C2 - 21561745
AN - SCOPUS:79957666012
VL - 86
SP - 409
EP - 413
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 2
ER -