Abstract
In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.
| Original language | English |
|---|---|
| Pages (from-to) | 409-413 |
| Number of pages | 5 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 86 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Sep 1 |
Fingerprint
Keywords
- Alginate
- Chitosan
- Fluorescence labeling
- Hydrogel
- Poly(γ-glutamic acid)
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan : Fluorescence observation of structure and cytocompatibility. / Lee, Yen Hsien; Chang, Jung Jhih; Lai, Wen Fu; Yang, Ming Chien; Chien, Chiang-Ting.
In: Colloids and Surfaces B: Biointerfaces, Vol. 86, No. 2, 01.09.2011, p. 409-413.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan
T2 - Fluorescence observation of structure and cytocompatibility
AU - Lee, Yen Hsien
AU - Chang, Jung Jhih
AU - Lai, Wen Fu
AU - Yang, Ming Chien
AU - Chien, Chiang-Ting
PY - 2011/9/1
Y1 - 2011/9/1
N2 - In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.
AB - In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.
KW - Alginate
KW - Chitosan
KW - Fluorescence labeling
KW - Hydrogel
KW - Poly(γ-glutamic acid)
UR - http://www.scopus.com/inward/record.url?scp=79957666012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957666012&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2011.04.002
DO - 10.1016/j.colsurfb.2011.04.002
M3 - Article
VL - 86
SP - 409
EP - 413
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 2
ER -