Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan: Fluorescence observation of structure and cytocompatibility

Yen Hsien Lee; Jung Jhih Chang; Wen Fu Lai; Ming Chien Yang; Chiang Ting Chien

doi:10.1016/j.colsurfb.2011.04.002

Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan: Fluorescence observation of structure and cytocompatibility

Yen Hsien Lee, Jung Jhih Chang, Wen Fu Lai, Ming Chien Yang, Chiang Ting Chien

Department of Life Science

Research output: Contribution to journal › Article

22 Citations (Scopus)

Abstract

In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca²⁺ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca²⁺ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.

Original language	English
Pages (from-to)	409-413
Number of pages	5
Journal	Colloids and Surfaces B: Biointerfaces
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/j.colsurfb.2011.04.002
Publication status	Published - 2011 Sep 1

Keywords

Alginate
Chitosan
Fluorescence labeling
Hydrogel
Poly(γ-glutamic acid)

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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Lee, Y. H., Chang, J. J., Lai, W. F., Yang, M. C., & Chien, C. T. (2011). Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan: Fluorescence observation of structure and cytocompatibility. Colloids and Surfaces B: Biointerfaces, 86(2), 409-413. https://doi.org/10.1016/j.colsurfb.2011.04.002

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