Layered hydrogel of poly(γ-glutamic acid), sodium alginate, and chitosan: Fluorescence observation of structure and cytocompatibility

Yen Hsien Lee, Jung Jhih Chang, Wen Fu Lai, Ming Chien Yang, Chiang Ting Chien

Research output: Contribution to journalArticle

22 Citations (Scopus)


In this study, a novel layered hydrogel composing of poly(γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.

Original languageEnglish
Pages (from-to)409-413
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Issue number2
Publication statusPublished - 2011 Sep 1



  • Alginate
  • Chitosan
  • Fluorescence labeling
  • Hydrogel
  • Poly(γ-glutamic acid)

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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