Large-volume sample sweeping with a high theoretical plate number using a coupled-capillary in capillary electrophoresis

Yen Sheng Chang; Chun Min Shih; Yen Cheng Li; Cheng Huang Lin

doi:10.2116/analsci.22.557

Large-volume sample sweeping with a high theoretical plate number using a coupled-capillary in capillary electrophoresis

Yen Sheng Chang, Chun Min Shih, Yen Cheng Li, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A large-volume sample injection (> 5 μL) with an extremely high theoretical plate number (N > 10⁷) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 μm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 10⁷ and 7.4 × 10⁶, respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10^-10 M (92 ppt) and 2.3 × 10^-10 M (83 ppt), respectively. 2006

Original language	English
Pages (from-to)	557-561
Number of pages	5
Journal	Analytical Sciences
Volume	22
Issue number	4
DOIs	https://doi.org/10.2116/analsci.22.557
Publication status	Published - 2006 Apr

ASJC Scopus subject areas

Analytical Chemistry

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title = "Large-volume sample sweeping with a high theoretical plate number using a coupled-capillary in capillary electrophoresis",

abstract = "A large-volume sample injection (> 5 μL) with an extremely high theoretical plate number (N > 107) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 μm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 107 and 7.4 × 106, respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10-10 M (92 ppt) and 2.3 × 10-10 M (83 ppt), respectively. 2006",

author = "Chang, {Yen Sheng} and Shih, {Chun Min} and Li, {Yen Cheng} and Lin, {Cheng Huang}",

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AU - Chang, Yen Sheng

AU - Shih, Chun Min

AU - Li, Yen Cheng

AU - Lin, Cheng Huang

PY - 2006/4

Y1 - 2006/4

N2 - A large-volume sample injection (> 5 μL) with an extremely high theoretical plate number (N > 107) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 μm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 107 and 7.4 × 106, respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10-10 M (92 ppt) and 2.3 × 10-10 M (83 ppt), respectively. 2006

AB - A large-volume sample injection (> 5 μL) with an extremely high theoretical plate number (N > 107) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 μm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 107 and 7.4 × 106, respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10-10 M (92 ppt) and 2.3 × 10-10 M (83 ppt), respectively. 2006

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Large-volume sample sweeping with a high theoretical plate number using a coupled-capillary in capillary electrophoresis

Abstract

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