Large-volume sample sweeping with a high theoretical plate number using a coupled-capillary in capillary electrophoresis

Yen Sheng Chang, Chun Min Shih, Yen Cheng Li, Cheng Huang Lin

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A large-volume sample injection (> 5 μL) with an extremely high theoretical plate number (N > 10 7 ) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 μm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 10 7 and 7.4 × 10 6 , respectively. The limits of detection (at S/N = 3) of these were determined to be 2.8 × 10 -10 M (92 ppt) and 2.3 × 10 -10 M (83 ppt), respectively. 2006

Original languageEnglish
Pages (from-to)557-561
Number of pages5
JournalAnalytical Sciences
Issue number4
Publication statusPublished - 2006 Apr


ASJC Scopus subject areas

  • Analytical Chemistry

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