Large-Scale Inhomogeneous Fluorescence Plasmonic Silver Chips: Origin and Mechanism

Liang Yan Hsu*, Hung Chi Yen, Ming Wei Lee, Yae Lin Sheu, Po Chun Chen, Hongjie Dai*, Chia Chun Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Large-scale inhomogeneous plasmonic metal chips have been demonstrated as a promising platform for biochemical sensing, but the origin of their strong fluorescence enhancements and average gap dependence is a challenging issue due to the complexity of modeling tremendous molecules within inhomogeneous gaps. To address this issue, we bridged microscopic mechanisms and macroscopic observations, developed a kinetic model, and experimentally investigated the fluorescence enhancement factors of IR800-streptavidin immobilized on metal nanoisland films (NIFs). Inspired by the kinetic model, we controlled the distribution of IR800-streptavidin within the valleys of NIFs by regioselective modification and achieved the fluorescence intensity enhancement up to 488-fold. The kinetic model allows us to qualitatively explain the mechanism of fluorescence intensity enhancements and quantitatively predict the trend of experimental enhancement factors, thereby determining the design principles of the plasmonic metal chips. Our study provides one key step further toward the sensing applications of large-scale plasmonic metal chips.

Original languageEnglish
Pages (from-to)3396-3408
Number of pages13
Issue number12
Publication statusPublished - 2020 Dec 3


  • Purcell effect
  • SDG3: Good health and well-being
  • biotin-streptavidin complex
  • chromophore
  • kinetic model
  • metal enhanced fluorescence
  • nanostructure
  • quantum yield
  • regioselective modification
  • sensor
  • spectroscopy

ASJC Scopus subject areas

  • General Chemistry
  • Biochemistry
  • Environmental Chemistry
  • General Chemical Engineering
  • Biochemistry, medical
  • Materials Chemistry


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