TY - JOUR
T1 - Large-scale expanded sample imaging with tiling lattice lightsheet microscopy
AU - Lu, Chieh Han
AU - Huang, Cheng Yu
AU - Tian, Xuejiao
AU - Chen, Peilin
AU - Chen, Bi Chang
N1 - Publisher Copyright:
© 2022
PY - 2023/1
Y1 - 2023/1
N2 - The ability to observe biological nanostructures forms a vital step in understanding their functions. Thanks to the invention of expansion microscopy (ExM) technology, super-resolution features of biological samples can now be easily visualized with conventional light microscopies. However, when the sample is physically expanded, the demand for deep and precise 3D imaging increases. Lattice lightsheet microscopy (LLSM), which utilizes a planar illumination that is confined within the imaging depth of high numerical aperture (NA=1.1) detection objective, fulfils such requirements. In addition, optical tiling could be implemented to increase the field of view (FoV) by moving the lightsheet without mechanically moving the samples or the objective for high-precision 3D imaging. In this review article, we will explain the principle of the tiling lattice lightsheet microscopy (tLLSM), which combines optical tiling and lattice lightsheet, and discuss the applications of tLLSM in ExM.
AB - The ability to observe biological nanostructures forms a vital step in understanding their functions. Thanks to the invention of expansion microscopy (ExM) technology, super-resolution features of biological samples can now be easily visualized with conventional light microscopies. However, when the sample is physically expanded, the demand for deep and precise 3D imaging increases. Lattice lightsheet microscopy (LLSM), which utilizes a planar illumination that is confined within the imaging depth of high numerical aperture (NA=1.1) detection objective, fulfils such requirements. In addition, optical tiling could be implemented to increase the field of view (FoV) by moving the lightsheet without mechanically moving the samples or the objective for high-precision 3D imaging. In this review article, we will explain the principle of the tiling lattice lightsheet microscopy (tLLSM), which combines optical tiling and lattice lightsheet, and discuss the applications of tLLSM in ExM.
KW - 3D imaging
KW - Expansion microscopy
KW - Lightsheet microscopy
KW - Super-resolution imaging
KW - Thick tissue imaging
KW - Tiling lattice lightsheet
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U2 - 10.1016/j.biocel.2022.106340
DO - 10.1016/j.biocel.2022.106340
M3 - Review article
C2 - 36442734
AN - SCOPUS:85144636368
SN - 1357-2725
VL - 154
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
M1 - 106340
ER -