Label-free three-dimensional cellular detection and analysis using holographic tomography and Raman tweezers spectroscopy

Chung Hsuan Huang; Han Yen Tu; Chau Jern Cheng

doi:10.1016/j.optlastec.2025.113346

Label-free three-dimensional cellular detection and analysis using holographic tomography and Raman tweezers spectroscopy

Chung Hsuan Huang
, Han Yen Tu^*
, Chau Jern Cheng

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This paper presents an innovative multimodal detection system that integrates holographic tomography with Raman tweezers spectroscopy for label-free cellular detection, providing three-dimensional (3D) cell morphology and biochemical composition analysis. Holographic tomography (HT) provides detailed 3D cell morphological images and spatial coordinates, while Raman tweezers spectroscopy (RTS) is employed to analyze biochemical characteristics within specific region of interest (ROI) based on a novel coordinate linking technique for effective connection between HT space and RTS space. The system achieves precise probing and detection with a positioning accuracy of 120 nm and an axial accuracy of 400 nm. Experimental results demonstrate its effectiveness in detecting and analyzing the 3D internal structures and the biochemical compositions of the human retinal pigment epithelial (ARPE-19) cells.

Original language	English
Article number	113346
Journal	Optics and Laser Technology
Volume	191
DOIs	https://doi.org/10.1016/j.optlastec.2025.113346
Publication status	Published - 2025 Dec

Keywords

Computer-generated hologram
Holographic Raman tweezers
Holographic tomography
Label-free imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

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10.1016/j.optlastec.2025.113346

Cite this

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title = "Label-free three-dimensional cellular detection and analysis using holographic tomography and Raman tweezers spectroscopy",

abstract = "This paper presents an innovative multimodal detection system that integrates holographic tomography with Raman tweezers spectroscopy for label-free cellular detection, providing three-dimensional (3D) cell morphology and biochemical composition analysis. Holographic tomography (HT) provides detailed 3D cell morphological images and spatial coordinates, while Raman tweezers spectroscopy (RTS) is employed to analyze biochemical characteristics within specific region of interest (ROI) based on a novel coordinate linking technique for effective connection between HT space and RTS space. The system achieves precise probing and detection with a positioning accuracy of 120 nm and an axial accuracy of 400 nm. Experimental results demonstrate its effectiveness in detecting and analyzing the 3D internal structures and the biochemical compositions of the human retinal pigment epithelial (ARPE-19) cells.",

keywords = "Computer-generated hologram, Holographic Raman tweezers, Holographic tomography, Label-free imaging",

author = "Huang, \{Chung Hsuan\} and Tu, \{Han Yen\} and Cheng, \{Chau Jern\}",

note = "Publisher Copyright: {\textcopyright} 2025 Elsevier Ltd",

year = "2025",

month = dec,

doi = "10.1016/j.optlastec.2025.113346",

language = "English",

volume = "191",

journal = "Optics and Laser Technology",

issn = "0030-3992",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Label-free three-dimensional cellular detection and analysis using holographic tomography and Raman tweezers spectroscopy

AU - Huang, Chung Hsuan

AU - Tu, Han Yen

AU - Cheng, Chau Jern

PY - 2025/12

Y1 - 2025/12

N2 - This paper presents an innovative multimodal detection system that integrates holographic tomography with Raman tweezers spectroscopy for label-free cellular detection, providing three-dimensional (3D) cell morphology and biochemical composition analysis. Holographic tomography (HT) provides detailed 3D cell morphological images and spatial coordinates, while Raman tweezers spectroscopy (RTS) is employed to analyze biochemical characteristics within specific region of interest (ROI) based on a novel coordinate linking technique for effective connection between HT space and RTS space. The system achieves precise probing and detection with a positioning accuracy of 120 nm and an axial accuracy of 400 nm. Experimental results demonstrate its effectiveness in detecting and analyzing the 3D internal structures and the biochemical compositions of the human retinal pigment epithelial (ARPE-19) cells.

AB - This paper presents an innovative multimodal detection system that integrates holographic tomography with Raman tweezers spectroscopy for label-free cellular detection, providing three-dimensional (3D) cell morphology and biochemical composition analysis. Holographic tomography (HT) provides detailed 3D cell morphological images and spatial coordinates, while Raman tweezers spectroscopy (RTS) is employed to analyze biochemical characteristics within specific region of interest (ROI) based on a novel coordinate linking technique for effective connection between HT space and RTS space. The system achieves precise probing and detection with a positioning accuracy of 120 nm and an axial accuracy of 400 nm. Experimental results demonstrate its effectiveness in detecting and analyzing the 3D internal structures and the biochemical compositions of the human retinal pigment epithelial (ARPE-19) cells.

KW - Computer-generated hologram

KW - Holographic Raman tweezers

KW - Holographic tomography

KW - Label-free imaging

UR - https://www.scopus.com/pages/publications/105007598724

UR - https://www.scopus.com/pages/publications/105007598724#tab=citedBy

U2 - 10.1016/j.optlastec.2025.113346

DO - 10.1016/j.optlastec.2025.113346

M3 - Article

AN - SCOPUS:105007598724

SN - 0030-3992

VL - 191

JO - Optics and Laser Technology

JF - Optics and Laser Technology

M1 - 113346

ER -

Label-free three-dimensional cellular detection and analysis using holographic tomography and Raman tweezers spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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