Juniperonic Acid Incorporation into the Phospholipids of Murine Macrophage Cells Modulates Pro-Inflammatory Mediator Production

Po Jung Tsai, Wen Cheng Huang, Shao Wei Lin, Sung Nien Chen, Hung Jing Shen, Hsiang Chang, Lu Te Chuang

Research output: Contribution to journalArticle

Abstract

Juniperonic acid (JPA; Δ5,11,14,17-20:4), originally identified in certain gymnosperm seeds, is a rare n-3 polyunsaturated fatty acid (PUFA) with lipid-modulating effects on rats and anti-proliferative effects on fibroblast cell proliferation. However, little is known how JPA exerted its immunosuppressive effect. The objective of this study was to investigate whether JPA inhibited the production of inflammatory mediators through the modulation of cellular phospholipid fatty acid compositions. Using standard lipid chemistry techniques in conjunction with argentated column chromatography, high-purity JPA (> 98%) was extracted, isolated, and purified from Biota kernels. When murine RAW264.7 macrophages were incubated with increasing concentrations of JPA, amounts of cellular phospholipid total PUFA, JPA, and Δ7-docosatetraenoic acid (Δ7-DTA; elongation product of JPA) increased in a dose-dependent manner; however, the proportions of total monounsaturated fatty acid (MUFA) and arachidonic acid (AA) decreased. JPA suppressed the production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) and the expression of inducible nitric oxide synthase (iNOS) up to 21, 75, 30, and 44%, respectively. The induction of cyclooxygenase-2 (COX-2) over-expression by JPA could account for the doubling of the PGE 2 level. Furthermore, JPA suppressed the expression of phosphorylated mitogen-activated protein kinases (MAPK). In a separate study using the mouse ear edema model, we demonstrated that JPA also significantly suppressed inflammation, as measured by ear thickness and biopsy weight. The anti-inflammatory properties of JPA could be due, in part, to the incorporation of JPA into cellular phospholipids with subsequent modulation of membrane-mediated MAPK signaling.

Original languageEnglish
Pages (from-to)1200-1214
Number of pages15
JournalInflammation
Volume41
Issue number4
DOIs
Publication statusPublished - 2018 Aug 1

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Phospholipids
Macrophages
Mitogen-Activated Protein Kinases
Ear
Gymnosperms
Lipids
Biota
Monounsaturated Fatty Acids
Omega-3 Fatty Acids
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Immunosuppressive Agents
Prostaglandins E
Unsaturated Fatty Acids
Arachidonic Acid
Chromatography
Interleukin-6
Edema
Seeds
Nitric Oxide

Keywords

  • Arachidonic acid (AA)
  • Juniperonic acid (JPA)
  • Macrophage
  • Prostaglandin E (PGE )
  • Type-2 cyclooxygenase (COX-2)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Juniperonic Acid Incorporation into the Phospholipids of Murine Macrophage Cells Modulates Pro-Inflammatory Mediator Production. / Tsai, Po Jung; Huang, Wen Cheng; Lin, Shao Wei; Chen, Sung Nien; Shen, Hung Jing; Chang, Hsiang; Chuang, Lu Te.

In: Inflammation, Vol. 41, No. 4, 01.08.2018, p. 1200-1214.

Research output: Contribution to journalArticle

Tsai, Po Jung ; Huang, Wen Cheng ; Lin, Shao Wei ; Chen, Sung Nien ; Shen, Hung Jing ; Chang, Hsiang ; Chuang, Lu Te. / Juniperonic Acid Incorporation into the Phospholipids of Murine Macrophage Cells Modulates Pro-Inflammatory Mediator Production. In: Inflammation. 2018 ; Vol. 41, No. 4. pp. 1200-1214.
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abstract = "Juniperonic acid (JPA; Δ5,11,14,17-20:4), originally identified in certain gymnosperm seeds, is a rare n-3 polyunsaturated fatty acid (PUFA) with lipid-modulating effects on rats and anti-proliferative effects on fibroblast cell proliferation. However, little is known how JPA exerted its immunosuppressive effect. The objective of this study was to investigate whether JPA inhibited the production of inflammatory mediators through the modulation of cellular phospholipid fatty acid compositions. Using standard lipid chemistry techniques in conjunction with argentated column chromatography, high-purity JPA (> 98{\%}) was extracted, isolated, and purified from Biota kernels. When murine RAW264.7 macrophages were incubated with increasing concentrations of JPA, amounts of cellular phospholipid total PUFA, JPA, and Δ7-docosatetraenoic acid (Δ7-DTA; elongation product of JPA) increased in a dose-dependent manner; however, the proportions of total monounsaturated fatty acid (MUFA) and arachidonic acid (AA) decreased. JPA suppressed the production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) and the expression of inducible nitric oxide synthase (iNOS) up to 21, 75, 30, and 44{\%}, respectively. The induction of cyclooxygenase-2 (COX-2) over-expression by JPA could account for the doubling of the PGE 2 level. Furthermore, JPA suppressed the expression of phosphorylated mitogen-activated protein kinases (MAPK). In a separate study using the mouse ear edema model, we demonstrated that JPA also significantly suppressed inflammation, as measured by ear thickness and biopsy weight. The anti-inflammatory properties of JPA could be due, in part, to the incorporation of JPA into cellular phospholipids with subsequent modulation of membrane-mediated MAPK signaling.",
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AU - Chuang, Lu Te

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