Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

Yu Cheng Hsu; Lucia L. Severinghaus; Yao Sung Lin; Shou Hsien Li

doi:10.1046/j.1471-8286.2003.00523.x

Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

Yu Cheng Hsu, Lucia L. Severinghaus, Yao Sung Lin, Shou Hsien Li^*

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.

Original language	English
Pages (from-to)	595-597
Number of pages	3
Journal	Molecular Ecology Notes
Volume	3
Issue number	4
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00523.x
Publication status	Published - 2003 Dec

Keywords

Enrichment
Genotyping
Lanyu scops owl
Microsatellites
Otus elegans botelensis
Strigidae

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry,Genetics and Molecular Biology

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10.1046/j.1471-8286.2003.00523.x

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abstract = "From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.",

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year = "2003",

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T1 - Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

AU - Hsu, Yu Cheng

AU - Severinghaus, Lucia L.

AU - Lin, Yao Sung

AU - Li, Shou Hsien

PY - 2003/12

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AB - From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.

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KW - Genotyping

KW - Lanyu scops owl

KW - Microsatellites

KW - Otus elegans botelensis

KW - Strigidae

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Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

Abstract

Keywords

ASJC Scopus subject areas

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