Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

Yu Cheng Hsu, Lucia L. Severinghaus, Yao Sung Lin, Shou Hsien Li

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy-Weinberg expectations.

Original languageEnglish
Pages (from-to)595-597
Number of pages3
JournalMolecular Ecology Notes
Volume3
Issue number4
DOIs
Publication statusPublished - 2003 Dec 1

Keywords

  • Enrichment
  • Genotyping
  • Lanyu scops owl
  • Microsatellites
  • Otus elegans botelensis
  • Strigidae

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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