Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

Yi Chun Chang, Shou Hsien Li, Ming Hsung Chang, Szu Lung Chen

Research output: Contribution to journalLetter

1 Citation (Scopus)

Abstract

Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999 %. These markers are useful not only in parentage assignment but also in population structure analysis.

Original languageEnglish
Pages (from-to)495-498
Number of pages4
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
Publication statusPublished - 2014 Jun

Fingerprint

Alligators and Crocodiles
crocodiles
parentage
Fresh Water
Microsatellite Repeats
microsatellite repeats
captive population
loci
genetic marker
wild population
Crocodylia
heterozygosity
Population
population structure
population size
allele
breeding
water
Population Density
Genetic Markers

Keywords

  • Crocodilian
  • False gharial
  • Gavialidae
  • Microsatellite

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii. / Chang, Yi Chun; Li, Shou Hsien; Chang, Ming Hsung; Chen, Szu Lung.

In: Conservation Genetics Resources, Vol. 6, No. 2, 06.2014, p. 495-498.

Research output: Contribution to journalLetter

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N2 - Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999 %. These markers are useful not only in parentage assignment but also in population structure analysis.

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