Isolation and characterization of 12 tetranucleotide repeat microsatellite loci from the green-backed tit (Parus monticolus)

Mei Ting Wang; Yu Cheng Hsu; Cheng Te Yao; Shou Hsien Li

doi:10.1111/j.1471-8286.2005.00955.x

Isolation and characterization of 12 tetranucleotide repeat microsatellite loci from the green-backed tit (Parus monticolus)

Mei Ting Wang, Yu Cheng Hsu, Cheng Te Yao, Shou Hsien Li^*

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

From a partial genomic library enriched for GATA short tandem repeats, we developed 12 polymorphic microsatellite loci from the green-backed tit (Parus monticolus). We characterized these loci by genotyping 30 adult individuals with unknown relationship. The number of alleles ranged from four to 17 per locus (mean = 9.3 alleles) and the observed heterozygosity for each locus ranged from 0.633 to 0.933 (mean = 0.789). All loci conformed to Hardy-Weinberg expectations. Four of 66 possible pairwise comparisons between loci showed significant gametic disequilibrium.

Original language	English
Pages (from-to)	439-442
Number of pages	4
Journal	Molecular Ecology Notes
Volume	5
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2005.00955.x
Publication status	Published - 2005 Jun

Keywords

Green-backed tit
Microsatellites
Parus monticolus

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry,Genetics and Molecular Biology

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10.1111/j.1471-8286.2005.00955.x

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abstract = "From a partial genomic library enriched for GATA short tandem repeats, we developed 12 polymorphic microsatellite loci from the green-backed tit (Parus monticolus). We characterized these loci by genotyping 30 adult individuals with unknown relationship. The number of alleles ranged from four to 17 per locus (mean = 9.3 alleles) and the observed heterozygosity for each locus ranged from 0.633 to 0.933 (mean = 0.789). All loci conformed to Hardy-Weinberg expectations. Four of 66 possible pairwise comparisons between loci showed significant gametic disequilibrium.",

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AU - Wang, Mei Ting

AU - Hsu, Yu Cheng

AU - Yao, Cheng Te

AU - Li, Shou Hsien

PY - 2005/6

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N2 - From a partial genomic library enriched for GATA short tandem repeats, we developed 12 polymorphic microsatellite loci from the green-backed tit (Parus monticolus). We characterized these loci by genotyping 30 adult individuals with unknown relationship. The number of alleles ranged from four to 17 per locus (mean = 9.3 alleles) and the observed heterozygosity for each locus ranged from 0.633 to 0.933 (mean = 0.789). All loci conformed to Hardy-Weinberg expectations. Four of 66 possible pairwise comparisons between loci showed significant gametic disequilibrium.

AB - From a partial genomic library enriched for GATA short tandem repeats, we developed 12 polymorphic microsatellite loci from the green-backed tit (Parus monticolus). We characterized these loci by genotyping 30 adult individuals with unknown relationship. The number of alleles ranged from four to 17 per locus (mean = 9.3 alleles) and the observed heterozygosity for each locus ranged from 0.633 to 0.933 (mean = 0.789). All loci conformed to Hardy-Weinberg expectations. Four of 66 possible pairwise comparisons between loci showed significant gametic disequilibrium.

KW - Green-backed tit

KW - Microsatellites

KW - Parus monticolus

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Isolation and characterization of 12 tetranucleotide repeat microsatellite loci from the green-backed tit (Parus monticolus)

Abstract

Keywords

ASJC Scopus subject areas

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