Isolation and characterization of 12 tetranucleotide repeat microsatellite loci from the green-backed tit (Parus monticolus)

Mei Ting Wang, Yu Cheng Hsu, Cheng Te Yao, Shou Hsien Li

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Abstract

From a partial genomic library enriched for GATA short tandem repeats, we developed 12 polymorphic microsatellite loci from the green-backed tit (Parus monticolus). We characterized these loci by genotyping 30 adult individuals with unknown relationship. The number of alleles ranged from four to 17 per locus (mean = 9.3 alleles) and the observed heterozygosity for each locus ranged from 0.633 to 0.933 (mean = 0.789). All loci conformed to Hardy-Weinberg expectations. Four of 66 possible pairwise comparisons between loci showed significant gametic disequilibrium.

Original languageEnglish
Pages (from-to)439-442
Number of pages4
JournalMolecular Ecology Notes
Volume5
Issue number2
DOIs
Publication statusPublished - 2005 Jun 1

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Keywords

  • Green-backed tit
  • Microsatellites
  • Parus monticolus

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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