Ionocyte distribution in gills of the euryhaline milkfish, Chanos chanos (Forsskål, 1775)

Chun Nian Chen, Li Yih Lin, Tsung Han Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As a marine euryhaline teleost, the milkfish, Chanos chanos, is considered a good model fish for studies of ionoregulation. In preparation for studies of the effects of seawater-freshwater adaptation on the biochemistry and morphology of the milkfish gill, the present investigation describes different distributions of ionocytes on the gill epithelium of seawater- versus freshwater-adapted fish. Paraffin sections of filaments revealed afferent, interlamellar, and efferent regions of the epithelium. Round eosinophilic epithelial cells (5-10 μm in diameter) were exhibited in the interlamellar epithelium near the afferent side of the filament. Due to their identical size and location, histochemical (osmium-zinc iodide) and immunofluorescent (Na, K-ATPase) staining was used to demonstrate that these eosinophilic, Na, K-ATPase-immunoreactive (NKIR) cells are ionocytes. It is thus hard to examine the apical openings of ionocytes by scanning electron microscopy in the gill epithelium of the milkfish as compared to those in the other teleosts, because most ionocytes are distributed in the interlamellar regions of the filaments. Further confocal micrographs showed abundant NKIR cells on the filamental epithelia of both seawater- and freshwater-adapted fish. However, NKIR cells were rarely observed on the lamellar epithelia of gills in seawater-adapted individuals, while they were commonly found in freshwater-adapted milkfish.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalZoological Studies
Volume43
Issue number4
Publication statusPublished - 2004 Oct
Externally publishedYes

Fingerprint

Chanos chanos
gills
sodium-potassium-exchanging ATPase
epithelium
seawater
fish
osmium
iodides
cells
biochemistry
alkanes
epithelial cells
scanning electron microscopy
zinc

Keywords

  • Euryhaline
  • Gill
  • K-ATPase
  • Milkfish
  • Mitochondria-rich cell
  • Na
  • Teleost

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Ionocyte distribution in gills of the euryhaline milkfish, Chanos chanos (Forsskål, 1775). / Chen, Chun Nian; Lin, Li Yih; Lee, Tsung Han.

In: Zoological Studies, Vol. 43, No. 4, 10.2004, p. 772-777.

Research output: Contribution to journalArticle

@article{c1f5c5308fe248429e2d19cfeedf0560,
title = "Ionocyte distribution in gills of the euryhaline milkfish, Chanos chanos (Forssk{\aa}l, 1775)",
abstract = "As a marine euryhaline teleost, the milkfish, Chanos chanos, is considered a good model fish for studies of ionoregulation. In preparation for studies of the effects of seawater-freshwater adaptation on the biochemistry and morphology of the milkfish gill, the present investigation describes different distributions of ionocytes on the gill epithelium of seawater- versus freshwater-adapted fish. Paraffin sections of filaments revealed afferent, interlamellar, and efferent regions of the epithelium. Round eosinophilic epithelial cells (5-10 μm in diameter) were exhibited in the interlamellar epithelium near the afferent side of the filament. Due to their identical size and location, histochemical (osmium-zinc iodide) and immunofluorescent (Na, K-ATPase) staining was used to demonstrate that these eosinophilic, Na, K-ATPase-immunoreactive (NKIR) cells are ionocytes. It is thus hard to examine the apical openings of ionocytes by scanning electron microscopy in the gill epithelium of the milkfish as compared to those in the other teleosts, because most ionocytes are distributed in the interlamellar regions of the filaments. Further confocal micrographs showed abundant NKIR cells on the filamental epithelia of both seawater- and freshwater-adapted fish. However, NKIR cells were rarely observed on the lamellar epithelia of gills in seawater-adapted individuals, while they were commonly found in freshwater-adapted milkfish.",
keywords = "Euryhaline, Gill, K-ATPase, Milkfish, Mitochondria-rich cell, Na, Teleost",
author = "Chen, {Chun Nian} and Lin, {Li Yih} and Lee, {Tsung Han}",
year = "2004",
month = "10",
language = "English",
volume = "43",
pages = "772--777",
journal = "Zoological Studies",
issn = "1021-5506",
publisher = "Biodiversity Research Center, Academia Sinica",
number = "4",

}

TY - JOUR

T1 - Ionocyte distribution in gills of the euryhaline milkfish, Chanos chanos (Forsskål, 1775)

AU - Chen, Chun Nian

AU - Lin, Li Yih

AU - Lee, Tsung Han

PY - 2004/10

Y1 - 2004/10

N2 - As a marine euryhaline teleost, the milkfish, Chanos chanos, is considered a good model fish for studies of ionoregulation. In preparation for studies of the effects of seawater-freshwater adaptation on the biochemistry and morphology of the milkfish gill, the present investigation describes different distributions of ionocytes on the gill epithelium of seawater- versus freshwater-adapted fish. Paraffin sections of filaments revealed afferent, interlamellar, and efferent regions of the epithelium. Round eosinophilic epithelial cells (5-10 μm in diameter) were exhibited in the interlamellar epithelium near the afferent side of the filament. Due to their identical size and location, histochemical (osmium-zinc iodide) and immunofluorescent (Na, K-ATPase) staining was used to demonstrate that these eosinophilic, Na, K-ATPase-immunoreactive (NKIR) cells are ionocytes. It is thus hard to examine the apical openings of ionocytes by scanning electron microscopy in the gill epithelium of the milkfish as compared to those in the other teleosts, because most ionocytes are distributed in the interlamellar regions of the filaments. Further confocal micrographs showed abundant NKIR cells on the filamental epithelia of both seawater- and freshwater-adapted fish. However, NKIR cells were rarely observed on the lamellar epithelia of gills in seawater-adapted individuals, while they were commonly found in freshwater-adapted milkfish.

AB - As a marine euryhaline teleost, the milkfish, Chanos chanos, is considered a good model fish for studies of ionoregulation. In preparation for studies of the effects of seawater-freshwater adaptation on the biochemistry and morphology of the milkfish gill, the present investigation describes different distributions of ionocytes on the gill epithelium of seawater- versus freshwater-adapted fish. Paraffin sections of filaments revealed afferent, interlamellar, and efferent regions of the epithelium. Round eosinophilic epithelial cells (5-10 μm in diameter) were exhibited in the interlamellar epithelium near the afferent side of the filament. Due to their identical size and location, histochemical (osmium-zinc iodide) and immunofluorescent (Na, K-ATPase) staining was used to demonstrate that these eosinophilic, Na, K-ATPase-immunoreactive (NKIR) cells are ionocytes. It is thus hard to examine the apical openings of ionocytes by scanning electron microscopy in the gill epithelium of the milkfish as compared to those in the other teleosts, because most ionocytes are distributed in the interlamellar regions of the filaments. Further confocal micrographs showed abundant NKIR cells on the filamental epithelia of both seawater- and freshwater-adapted fish. However, NKIR cells were rarely observed on the lamellar epithelia of gills in seawater-adapted individuals, while they were commonly found in freshwater-adapted milkfish.

KW - Euryhaline

KW - Gill

KW - K-ATPase

KW - Milkfish

KW - Mitochondria-rich cell

KW - Na

KW - Teleost

UR - http://www.scopus.com/inward/record.url?scp=12244249492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12244249492&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 772

EP - 777

JO - Zoological Studies

JF - Zoological Studies

SN - 1021-5506

IS - 4

ER -