Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors

You Ren Hsu, Geng Yen Lee, Jen Inn Chyi, Chung Ke Chang, Chih Cheng Huang, Chen Pin Hsu, Tai-huang Huang, Fan Ren, Yu Lin Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this article, we used AlGaN/GaN high electron mobility transistors (HEMTs) to construct the first label-free semiconductor-sensor-based binding assay to our knowledge. Our results suggested that the nucleotide-c terminal domain of SARS coronavirus (SARS-CoV) nucleocapsid protein interaction is a two-step binding event with two dissociation constants (Kd1 = 0.052 nM, and Kd2 = 51.24nM) extracted by using the modified two-binding-site Langmuir isotherm equation proposed here. We found that there were at least two protein binding sites on the specific 41-base SARS-CoV double-stranded DNA (dsDNA) genome (29,580-29621) conjugated with a 20-mer poly-dT tail. This result presented a high binding affinity is comparable with the antibody-antigen reaction, and suggested this designed dsDNA could be treated as an aptamer for SARS-CoV N protein capture.

Original languageEnglish
Title of host publicationIEEE SENSORS 2012 - Proceedings
DOIs
Publication statusPublished - 2012 Dec 1
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan
Duration: 2012 Oct 282012 Oct 31

Publication series

NameProceedings of IEEE Sensors

Other

Other11th IEEE SENSORS 2012 Conference
CountryTaiwan
CityTaipei
Period12/10/2812/10/31

Fingerprint

High electron mobility transistors
Binding sites
Nucleotides
Antigen-antibody reactions
Proteins
Isotherms
Labels
Assays
DNA
Genes
Semiconductor materials
Sensors
Protein Binding

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Hsu, Y. R., Lee, G. Y., Chyi, J. I., Chang, C. K., Huang, C. C., Hsu, C. P., ... Wang, Y. L. (2012). Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors. In IEEE SENSORS 2012 - Proceedings [6411277] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2012.6411277

Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors. / Hsu, You Ren; Lee, Geng Yen; Chyi, Jen Inn; Chang, Chung Ke; Huang, Chih Cheng; Hsu, Chen Pin; Huang, Tai-huang; Ren, Fan; Wang, Yu Lin.

IEEE SENSORS 2012 - Proceedings. 2012. 6411277 (Proceedings of IEEE Sensors).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hsu, YR, Lee, GY, Chyi, JI, Chang, CK, Huang, CC, Hsu, CP, Huang, T, Ren, F & Wang, YL 2012, Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors. in IEEE SENSORS 2012 - Proceedings., 6411277, Proceedings of IEEE Sensors, 11th IEEE SENSORS 2012 Conference, Taipei, Taiwan, 12/10/28. https://doi.org/10.1109/ICSENS.2012.6411277
Hsu YR, Lee GY, Chyi JI, Chang CK, Huang CC, Hsu CP et al. Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors. In IEEE SENSORS 2012 - Proceedings. 2012. 6411277. (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2012.6411277
Hsu, You Ren ; Lee, Geng Yen ; Chyi, Jen Inn ; Chang, Chung Ke ; Huang, Chih Cheng ; Hsu, Chen Pin ; Huang, Tai-huang ; Ren, Fan ; Wang, Yu Lin. / Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors. IEEE SENSORS 2012 - Proceedings. 2012. (Proceedings of IEEE Sensors).
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