Interaction Analysis Between Polyglutamine-expanded TATA Box Binding Protein (TBP) and High Mobility Group Box 1 (HMGB1)

楊 淑婷(Shu-Ting Yang), 陳 襄銘(Hsiang-Ming Chen), 李 麗卿(Li-Ching Lee), 黃 詩涵(Shih-Han Huang), 林 志信(Chih-Hsin Lin), 李 冠群(Guan-Chiun Lee), 李 振綱(Cheng-Kang Lee), 李 桂楨(Guey-Jen Lee-Chen)

Research output: Contribution to journalArticle

Abstract

第十七型脊髓小腦共濟失調症(SCA17)與轉錄起始因子TATA binding protein (TBP)基因上的多麩醯胺擴增相關。TBP與包括high mobility group box 1 (HMGB1)在內的其他蛋白因子交互作用,來調節基因的表現。本研究藉重組的HMGB1 及TBP/Q20~61 N端蛋白,探討TBP Q長度是否影響其與HMGB1的結合。首先共表現HMGB1及TBP於 HEK-293細胞後,利用免疫共沉澱及GST pull-down試驗,確認HMGB1 與TBP在細胞內的結合。其次原核E. coli表現的重組HMGB1 及TBP/Q20~61蛋白的GSTpull-down試驗,亦顯示HMGB1與TBP的結合。最後即時石英晶體微量天平(QCM)試驗顯示,HMGB1-TBP交互作用隨TBP蛋白上polyQ長度的增加而減弱。綜合先前報導的HMGB1-TBP交互作用可增強TBP結合TATA速率及穩定性,我們的研究顯示多麩醯胺擴增TBP與HMGB1結合的減弱,可能與SCA17致病機轉相關。
Original languageChinese
Pages (from-to)17-26
Number of pages10
JournalBio Formosa
Volume48
DOIs
Publication statusPublished - 2013

Keywords

  • 免疫共沉澱
  • 石英晶體微量天平
  • TBP
  • HMGB1
  • co-IP
  • GST pull-down
  • QCM

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    楊淑婷(Shu-Ting Y, 陳襄銘(Hsiang-Ming C, 李麗卿(Li-Ching L, 黃詩涵(Shih-Han H, 林志信(Chih-Hsin L, 李冠群(Guan-Chiun L, 李振綱(Cheng-Kang L, & 李桂楨(Guey-Jen L-C (2013). Interaction Analysis Between Polyglutamine-expanded TATA Box Binding Protein (TBP) and High Mobility Group Box 1 (HMGB1). Bio Formosa, 48, 17-26. https://doi.org/10.6248/BF.2013.48.03