Insulin-like growth factor-I is an autocrine regulator for the brain metastatic variant of a human non-small cell lung cell line

Chiu Chin Hwang; Kang Fang; Limin Li; Stephen H. Shih

doi:10.1016/0304-3835(95)03845-N

Insulin-like growth factor-I is an autocrine regulator for the brain metastatic variant of a human non-small cell lung cell line

Chiu Chin Hwang, Kang Fang^*, Limin Li, Stephen H. Shih

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Insulin-like growth factor (IGF-I) is associated with autocrine and paracrine stimulation for cell growth and development of brain tumor cells. The function of IGF-I in the brain metastatic variant of human lung cancer cells is investigated. The cells used here were derived in vivo with intracarotid injection of human non-small cell lung carcinoma NCI-H226. The tumor was developed as a cultured cell line, H226Br. Unlike the parental cells, H226Br was tumorigenic in nu/nu nude mice. Reverse transcriptase-polymerase chain reaction showed that IGF-I transcript of H226Br is increased compared to that of parental cells. The amount of IGF-I secreted in cultured medium of H226Br is higher than that of cultured parental cells. The IGF-I receptor-specific antibody, αIR3, inhibits H226Br growth in serum-free culture. The results established that IGF-I is an autocrine growth regulator for human non-small cell lung cancer cells that progressed to brain.

Original language	English
Pages (from-to)	157-163
Number of pages	7
Journal	Cancer Letters
Volume	94
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(95)03845-N
Publication status	Published - 1995 Aug 1

Keywords

Autocrine
Insulin-like growth factor-I
Metastasis

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(95)03845-N

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title = "Insulin-like growth factor-I is an autocrine regulator for the brain metastatic variant of a human non-small cell lung cell line",

abstract = "Insulin-like growth factor (IGF-I) is associated with autocrine and paracrine stimulation for cell growth and development of brain tumor cells. The function of IGF-I in the brain metastatic variant of human lung cancer cells is investigated. The cells used here were derived in vivo with intracarotid injection of human non-small cell lung carcinoma NCI-H226. The tumor was developed as a cultured cell line, H226Br. Unlike the parental cells, H226Br was tumorigenic in nu/nu nude mice. Reverse transcriptase-polymerase chain reaction showed that IGF-I transcript of H226Br is increased compared to that of parental cells. The amount of IGF-I secreted in cultured medium of H226Br is higher than that of cultured parental cells. The IGF-I receptor-specific antibody, αIR3, inhibits H226Br growth in serum-free culture. The results established that IGF-I is an autocrine growth regulator for human non-small cell lung cancer cells that progressed to brain.",

keywords = "Autocrine, Insulin-like growth factor-I, Metastasis",

author = "Hwang, {Chiu Chin} and Kang Fang and Limin Li and Shih, {Stephen H.}",

note = "Funding Information: We are indebtedt o Dr. I.J. Frdler of the Department tit{\textquoteright} Cell Biology, M.D. Anderson Cancer Center, Houston. Texas for his assistancein cell line development. Partial support from the grant of the National Science Council, Executive Yuan, Republic of China (NSC-83-021 l-BOO3-008)i s appreciated",

year = "1995",

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doi = "10.1016/0304-3835(95)03845-N",

language = "English",

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journal = "Cancer Letters",

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T1 - Insulin-like growth factor-I is an autocrine regulator for the brain metastatic variant of a human non-small cell lung cell line

AU - Hwang, Chiu Chin

AU - Fang, Kang

AU - Li, Limin

AU - Shih, Stephen H.

N1 - Funding Information: We are indebtedt o Dr. I.J. Frdler of the Department tit’ Cell Biology, M.D. Anderson Cancer Center, Houston. Texas for his assistancein cell line development. Partial support from the grant of the National Science Council, Executive Yuan, Republic of China (NSC-83-021 l-BOO3-008)i s appreciated

PY - 1995/8/1

Y1 - 1995/8/1

N2 - Insulin-like growth factor (IGF-I) is associated with autocrine and paracrine stimulation for cell growth and development of brain tumor cells. The function of IGF-I in the brain metastatic variant of human lung cancer cells is investigated. The cells used here were derived in vivo with intracarotid injection of human non-small cell lung carcinoma NCI-H226. The tumor was developed as a cultured cell line, H226Br. Unlike the parental cells, H226Br was tumorigenic in nu/nu nude mice. Reverse transcriptase-polymerase chain reaction showed that IGF-I transcript of H226Br is increased compared to that of parental cells. The amount of IGF-I secreted in cultured medium of H226Br is higher than that of cultured parental cells. The IGF-I receptor-specific antibody, αIR3, inhibits H226Br growth in serum-free culture. The results established that IGF-I is an autocrine growth regulator for human non-small cell lung cancer cells that progressed to brain.

AB - Insulin-like growth factor (IGF-I) is associated with autocrine and paracrine stimulation for cell growth and development of brain tumor cells. The function of IGF-I in the brain metastatic variant of human lung cancer cells is investigated. The cells used here were derived in vivo with intracarotid injection of human non-small cell lung carcinoma NCI-H226. The tumor was developed as a cultured cell line, H226Br. Unlike the parental cells, H226Br was tumorigenic in nu/nu nude mice. Reverse transcriptase-polymerase chain reaction showed that IGF-I transcript of H226Br is increased compared to that of parental cells. The amount of IGF-I secreted in cultured medium of H226Br is higher than that of cultured parental cells. The IGF-I receptor-specific antibody, αIR3, inhibits H226Br growth in serum-free culture. The results established that IGF-I is an autocrine growth regulator for human non-small cell lung cancer cells that progressed to brain.

KW - Autocrine

KW - Insulin-like growth factor-I

KW - Metastasis

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Insulin-like growth factor-I is an autocrine regulator for the brain metastatic variant of a human non-small cell lung cell line

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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