Insulin-like growth factor-I is an autocrine regulator for the brain metastatic variant of a human non-small cell lung cell line

Chiu Chin Hwang, Kang Fang, Limin Li, Stephen H. Shih

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Insulin-like growth factor (IGF-I) is associated with autocrine and paracrine stimulation for cell growth and development of brain tumor cells. The function of IGF-I in the brain metastatic variant of human lung cancer cells is investigated. The cells used here were derived in vivo with intracarotid injection of human non-small cell lung carcinoma NCI-H226. The tumor was developed as a cultured cell line, H226Br. Unlike the parental cells, H226Br was tumorigenic in nu/nu nude mice. Reverse transcriptase-polymerase chain reaction showed that IGF-I transcript of H226Br is increased compared to that of parental cells. The amount of IGF-I secreted in cultured medium of H226Br is higher than that of cultured parental cells. The IGF-I receptor-specific antibody, αIR3, inhibits H226Br growth in serum-free culture. The results established that IGF-I is an autocrine growth regulator for human non-small cell lung cancer cells that progressed to brain.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalCancer Letters
Volume94
Issue number2
DOIs
Publication statusPublished - 1995 Aug 1

Keywords

  • Autocrine
  • Insulin-like growth factor-I
  • Metastasis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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