Inhibition of Escherichia coli respiratory enzymes by short visible femtosecond laser irradiation

Chieh Han Lu, Kung Hsuan Lin, Yung-Yuan Hsu, Kong Thon Tsen, Yung Shu Kuan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A visible femtosecond laser is shown to be capable of selectively inactivating a wide spectrum of microorganisms in a wavelength and pulse width dependent manner. However, the mechanism of how a visible femtosecond laser affects the viability of different microorganisms is still elusive. In this paper, the cellular surface properties, membrane integrity and metabolic rate of Escherichia coli (E. coli) irradiated by a visible femtosecond laser (λ=415nm, pulse width=100fs) with different exposure times were investigated. Our results showed that femtosecond laser treatment for 60min led to cytoplasmic leakage, protein aggregation and alternation of the physical properties of the E. coli cell membrane. In comparison, a 10min exposure of bacteria to femtosecond laser irradiation induced an immediate reduction of 75% in the glucose-dependent respiratory rate, while the cytoplasmic leakage was not detected. Results from enzymatic assays showed that oxidases and dehydrogenases involved in the E. coli respiratory chain exhibited divergent susceptibility after laser irradiation. This early commencement of respiratory inhibition after a short irradiation is presumed to have a dominant effect on the early stage of bacteria inactivation.

Original languageEnglish
Article number315402
JournalJournal of Physics D: Applied Physics
Volume47
Issue number31
DOIs
Publication statusPublished - 2014 Aug 6

Fingerprint

Enzyme inhibition
Laser beam effects
Escherichia
Ultrashort pulses
Escherichia coli
enzymes
Enzymes
irradiation
lasers
Microorganisms
microorganisms
Bacteria
Oxidoreductases
bacteria
pulse duration
respiratory rate
leakage
Cell membranes
dehydrogenases
oxidase

Keywords

  • bacteria inactivation
  • femtosecond lasers
  • respiratory inhibition

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Cite this

Inhibition of Escherichia coli respiratory enzymes by short visible femtosecond laser irradiation. / Lu, Chieh Han; Lin, Kung Hsuan; Hsu, Yung-Yuan; Tsen, Kong Thon; Kuan, Yung Shu.

In: Journal of Physics D: Applied Physics, Vol. 47, No. 31, 315402, 06.08.2014.

Research output: Contribution to journalArticle

Lu, Chieh Han ; Lin, Kung Hsuan ; Hsu, Yung-Yuan ; Tsen, Kong Thon ; Kuan, Yung Shu. / Inhibition of Escherichia coli respiratory enzymes by short visible femtosecond laser irradiation. In: Journal of Physics D: Applied Physics. 2014 ; Vol. 47, No. 31.
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