Influence of Yokukansan on the refractive index of neuroblastoma cells

Maria Baczewska; Milena Królikowska; Martyna Mazur; Natalia Nowak; Jędrzej Szymański; Wojciech Krauze; Chau Jern Cheng; Małgorzata KujawińSka

doi:10.1364/BOE.481169

Influence of Yokukansan on the refractive index of neuroblastoma cells

Maria Baczewska^*, Milena Królikowska, Martyna Mazur, Natalia Nowak, Jędrzej Szymański, Wojciech Krauze, Chau Jern Cheng, Małgorzata KujawińSka

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Yokukansan (YKS) is a traditional Japanese herbal medicine that is increasingly being studied for its effects on neurodegenerative diseases. In our study, we presented a novel methodology for a multimodal analysis of the effects of YKS on nerve cells. The measurements of 3D refractive index distribution and its changes performed by holographic tomography were supported with an investigation by Raman micro-spectroscopy and fluorescence microscopy to gather complementary morphological and chemical information about cells and YKS influence. It was shown that at the concentrations tested, YKS inhibits proliferation, possibly involving reactive oxygen species. Also substantial changes in the cell RI after few hours of YKS exposure were detected, followed by longer-term changes in cell lipid composition and chromatin state.

Original language	English
Pages (from-to)	1959-1973
Number of pages	15
Journal	Biomedical Optics Express
Volume	14
Issue number	5
DOIs	https://doi.org/10.1364/BOE.481169
Publication status	Published - 2023 May 1

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

Access to Document

10.1364/BOE.481169

Cite this

@article{360dec0f2a8b4dc6ad46b462b1c49b76,

title = "Influence of Yokukansan on the refractive index of neuroblastoma cells",

abstract = "Yokukansan (YKS) is a traditional Japanese herbal medicine that is increasingly being studied for its effects on neurodegenerative diseases. In our study, we presented a novel methodology for a multimodal analysis of the effects of YKS on nerve cells. The measurements of 3D refractive index distribution and its changes performed by holographic tomography were supported with an investigation by Raman micro-spectroscopy and fluorescence microscopy to gather complementary morphological and chemical information about cells and YKS influence. It was shown that at the concentrations tested, YKS inhibits proliferation, possibly involving reactive oxygen species. Also substantial changes in the cell RI after few hours of YKS exposure were detected, followed by longer-term changes in cell lipid composition and chromatin state.",

author = "Maria Baczewska and Milena Kr{\'o}likowska and Martyna Mazur and Natalia Nowak and J{\c e}drzej Szyma{\'n}ski and Wojciech Krauze and Cheng, {Chau Jern} and Ma{\l}gorzata Kujawi{\'n}Ska",

note = "Publisher Copyright: {\textcopyright} 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.",

year = "2023",

month = may,

day = "1",

doi = "10.1364/BOE.481169",

language = "English",

volume = "14",

pages = "1959--1973",

journal = "Biomedical Optics Express",

issn = "2156-7085",

publisher = "Optica Publishing Group (formerly OSA)",

number = "5",

}

TY - JOUR

T1 - Influence of Yokukansan on the refractive index of neuroblastoma cells

AU - Baczewska, Maria

AU - Królikowska, Milena

AU - Mazur, Martyna

AU - Nowak, Natalia

AU - Szymański, Jędrzej

AU - Krauze, Wojciech

AU - Cheng, Chau Jern

AU - KujawińSka, Małgorzata

PY - 2023/5/1

Y1 - 2023/5/1

N2 - Yokukansan (YKS) is a traditional Japanese herbal medicine that is increasingly being studied for its effects on neurodegenerative diseases. In our study, we presented a novel methodology for a multimodal analysis of the effects of YKS on nerve cells. The measurements of 3D refractive index distribution and its changes performed by holographic tomography were supported with an investigation by Raman micro-spectroscopy and fluorescence microscopy to gather complementary morphological and chemical information about cells and YKS influence. It was shown that at the concentrations tested, YKS inhibits proliferation, possibly involving reactive oxygen species. Also substantial changes in the cell RI after few hours of YKS exposure were detected, followed by longer-term changes in cell lipid composition and chromatin state.

AB - Yokukansan (YKS) is a traditional Japanese herbal medicine that is increasingly being studied for its effects on neurodegenerative diseases. In our study, we presented a novel methodology for a multimodal analysis of the effects of YKS on nerve cells. The measurements of 3D refractive index distribution and its changes performed by holographic tomography were supported with an investigation by Raman micro-spectroscopy and fluorescence microscopy to gather complementary morphological and chemical information about cells and YKS influence. It was shown that at the concentrations tested, YKS inhibits proliferation, possibly involving reactive oxygen species. Also substantial changes in the cell RI after few hours of YKS exposure were detected, followed by longer-term changes in cell lipid composition and chromatin state.

UR - http://www.scopus.com/inward/record.url?scp=85174340297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85174340297&partnerID=8YFLogxK

U2 - 10.1364/BOE.481169

DO - 10.1364/BOE.481169

M3 - Article

AN - SCOPUS:85174340297

SN - 2156-7085

VL - 14

SP - 1959

EP - 1973

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 5

ER -

Influence of Yokukansan on the refractive index of neuroblastoma cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this