Induction of phosphoenolpyruvate carboxykinase (PEPCK) during acute acidosis and its role in acid secretion by V-ATPase-expressing ionocytes

Fumiya Furukawa, Yung-Che Tseng, Sian Tai Liu, Yi Ling Chou, Ching Chun Lin, Po Hsuan Sung, Katsuhisa Uchida, Li-Yih Lin, Pung Pung Hwang

Research output: Contribution to journalArticle

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Abstract

Vacuolar-Type H+-ATPase (V-ATPase) takes the central role in pumping H+ through cell membranes of diverse organisms, which is essential for surviving acid-base fluctuating lifestyles or environments. In mammals, although glucose is believed to be an important energy source to drive V-ATPase, and phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme for gluconeogenesis, is known to be activated in response to acidosis, the link between acid secretion and PEPCK activation remains unclear. In the present study, we used zebrafish larva as an in vivo model to show the role of acid-inducible PEPCK activity in glucose production to support higher rate of H+ secretion via V-ATPase, by utilizing gene knockdown, glucose supplementation, and non-invasive scanning ion-selective electrode technique (SIET). Zebrafish larvae increased V-ATPase-mediated acid secretion and transiently expression of Pck1, a zebrafish homolog of PEPCK, in response to acid stress. When pck1 gene was knocked down by specific morpholino, the H+ secretion via V-ATPase decreased, but this effect was rescued by supplementation of glucose into the yolk. By assessing changes in amino acid content and gene expression of respective enzymes, glutamine and glutamate appeared to be the major source for replenishment of Krebs cycle intermediates, which are subtracted by Pck1 activity. Unexpectedly, pck1 knockdown did not affect glutamine/glutamate catalysis, which implies that Pck1 does not necessarily drive this process. The present study provides the first in vivo evidence that acid-induced PEPCK provides glucose for acid-base homeostasis at an individual level, which is supported by rapid pumping of H+ via V-ATPase at the cellular level.

Original languageEnglish
Pages (from-to)712-725
Number of pages14
JournalInternational Journal of Biological Sciences
Volume11
Issue number6
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

Vacuolar Proton-Translocating ATPases
Phosphoenolpyruvate
H-transporting ATP synthase
acidosis
Acidosis
secretion
Acids
glucose
acids
acid
Glucose
Zebrafish
Danio rerio
Glutamine
glutamine
glutamates
Larva
Glutamic Acid
pumping
specific ion electrodes

Keywords

  • Acid-base regulation
  • Gluconeogenesis
  • Glutamine
  • PEPCK
  • V-ATPase

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Induction of phosphoenolpyruvate carboxykinase (PEPCK) during acute acidosis and its role in acid secretion by V-ATPase-expressing ionocytes. / Furukawa, Fumiya; Tseng, Yung-Che; Liu, Sian Tai; Chou, Yi Ling; Lin, Ching Chun; Sung, Po Hsuan; Uchida, Katsuhisa; Lin, Li-Yih; Hwang, Pung Pung.

In: International Journal of Biological Sciences, Vol. 11, No. 6, 01.05.2015, p. 712-725.

Research output: Contribution to journalArticle

Furukawa, Fumiya ; Tseng, Yung-Che ; Liu, Sian Tai ; Chou, Yi Ling ; Lin, Ching Chun ; Sung, Po Hsuan ; Uchida, Katsuhisa ; Lin, Li-Yih ; Hwang, Pung Pung. / Induction of phosphoenolpyruvate carboxykinase (PEPCK) during acute acidosis and its role in acid secretion by V-ATPase-expressing ionocytes. In: International Journal of Biological Sciences. 2015 ; Vol. 11, No. 6. pp. 712-725.
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