Independency of Fe ions in hemoglobin on immunomagnetic reduction assay

S. Y. Yang; C. B. Lan; C. H. Chen; H. E. Horng; Chin Yih Hong; H. C. Yang; Y. K. Lai; Y. H. Lin; K. S. Teng

doi:10.1016/j.jmmm.2009.05.075

Independency of Fe ions in hemoglobin on immunomagnetic reduction assay

S. Y. Yang, C. B. Lan, C. H. Chen, H. E. Horng^*, Chin Yih Hong, H. C. Yang, Y. K. Lai, Y. H. Lin, K. S. Teng

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Immunomagnetic reduction (IMR), which involves measuring the reduction in the ac magnetic susceptibility of magnetic reagents, is due to the association between bio-functionalized magnetic nanoparticles and target bio-molecules. This has been demonstrated for assaying proteins in solutions free of Fe ions, such as serum. In this work, the validity of IMR assay for samples rich in Fe ions like hemoglobin (Hb) is investigated. According to the results, there is no magnetic signal contributed by Fe-ion-rich Hb. Furthermore, the results show a high sensitivity in assaying hemoglobin A1c (HbA1c) by using IMR.

Original language	English
Pages (from-to)	3266-3269
Number of pages	4
Journal	Journal of Magnetism and Magnetic Materials
Volume	321
Issue number	19
DOIs	https://doi.org/10.1016/j.jmmm.2009.05.075
Publication status	Published - 2009 Oct

Keywords

Hemoglobin
Immunomagnetic reduction
Magnetic nanoparticle

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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10.1016/j.jmmm.2009.05.075

Cite this

@article{004bdcf3c4ba4949a72cc8178d0c0ec8,

title = "Independency of Fe ions in hemoglobin on immunomagnetic reduction assay",

abstract = "Immunomagnetic reduction (IMR), which involves measuring the reduction in the ac magnetic susceptibility of magnetic reagents, is due to the association between bio-functionalized magnetic nanoparticles and target bio-molecules. This has been demonstrated for assaying proteins in solutions free of Fe ions, such as serum. In this work, the validity of IMR assay for samples rich in Fe ions like hemoglobin (Hb) is investigated. According to the results, there is no magnetic signal contributed by Fe-ion-rich Hb. Furthermore, the results show a high sensitivity in assaying hemoglobin A1c (HbA1c) by using IMR.",

keywords = "Hemoglobin, Immunomagnetic reduction, Magnetic nanoparticle",

author = "Yang, {S. Y.} and Lan, {C. B.} and Chen, {C. H.} and Horng, {H. E.} and Hong, {Chin Yih} and Yang, {H. C.} and Lai, {Y. K.} and Lin, {Y. H.} and Teng, {K. S.}",

note = "Funding Information: This work is supported by the National Science Council of Taiwan under Grant numbers NSC97-2112-M-003-012 and NSC98-2752-M-002-016-PAE, and by Ministry of Economic Affairs of Taiwan under Grant numbers 1Z970688 (SBIR) and S09800226-203 (JAID).",

year = "2009",

month = oct,

doi = "10.1016/j.jmmm.2009.05.075",

language = "English",

volume = "321",

pages = "3266--3269",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

publisher = "Elsevier",

number = "19",

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TY - JOUR

T1 - Independency of Fe ions in hemoglobin on immunomagnetic reduction assay

AU - Yang, S. Y.

AU - Lan, C. B.

AU - Chen, C. H.

AU - Horng, H. E.

AU - Hong, Chin Yih

AU - Yang, H. C.

AU - Lai, Y. K.

AU - Lin, Y. H.

AU - Teng, K. S.

N1 - Funding Information: This work is supported by the National Science Council of Taiwan under Grant numbers NSC97-2112-M-003-012 and NSC98-2752-M-002-016-PAE, and by Ministry of Economic Affairs of Taiwan under Grant numbers 1Z970688 (SBIR) and S09800226-203 (JAID).

PY - 2009/10

Y1 - 2009/10

N2 - Immunomagnetic reduction (IMR), which involves measuring the reduction in the ac magnetic susceptibility of magnetic reagents, is due to the association between bio-functionalized magnetic nanoparticles and target bio-molecules. This has been demonstrated for assaying proteins in solutions free of Fe ions, such as serum. In this work, the validity of IMR assay for samples rich in Fe ions like hemoglobin (Hb) is investigated. According to the results, there is no magnetic signal contributed by Fe-ion-rich Hb. Furthermore, the results show a high sensitivity in assaying hemoglobin A1c (HbA1c) by using IMR.

AB - Immunomagnetic reduction (IMR), which involves measuring the reduction in the ac magnetic susceptibility of magnetic reagents, is due to the association between bio-functionalized magnetic nanoparticles and target bio-molecules. This has been demonstrated for assaying proteins in solutions free of Fe ions, such as serum. In this work, the validity of IMR assay for samples rich in Fe ions like hemoglobin (Hb) is investigated. According to the results, there is no magnetic signal contributed by Fe-ion-rich Hb. Furthermore, the results show a high sensitivity in assaying hemoglobin A1c (HbA1c) by using IMR.

KW - Hemoglobin

KW - Immunomagnetic reduction

KW - Magnetic nanoparticle

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UR - http://www.scopus.com/inward/citedby.url?scp=67650099781&partnerID=8YFLogxK

U2 - 10.1016/j.jmmm.2009.05.075

DO - 10.1016/j.jmmm.2009.05.075

M3 - Article

AN - SCOPUS:67650099781

SN - 0304-8853

VL - 321

SP - 3266

EP - 3269

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 19

ER -

Independency of Fe ions in hemoglobin on immunomagnetic reduction assay

Abstract

Keywords

ASJC Scopus subject areas

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