In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel)

Y. S. Lai; H. C. Chiu; S. Murali; I. C. Guo; S. C. Chen; K. Fang; C. Y. Chang

doi:10.1046/j.1365-2761.2001.00293.x

In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel)

Y. S. Lai, H. C. Chiu, S. Murali, I. C. Guo, S. C. Chen, K. Fang, C. Y. Chang^*

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Mouse monoclonal antibodies (MAbs) were produced by using yellow grouper nervous necrosis virus (YGNNV) as an immunogen, isolated from infected yellow grouper, Epinephelus awoara (Temminck & Schlegel), and propagated in GB cells. In enzyme linked immunosorbent assay (ELISA), 43 hybridoma clones secreting MAbs strongly reacted with the purified virus. Ten of them showed a higher neutralization index (NI) value between 6.5 and 4.5 (log₁₀ NI) than the other 33 MAbs against YGNNV infection in cell culture. All 10 MAbs belonged to the IgG isotype with a K light chain and recognized the 42 kDa coat protein of YGNNV by Western blot analysis. Immunohistochemical results demonstrated that the viral signals co-located with pathological lesions observed in retina, brain and spinal cord. These results indicate that the MAbs are useful for confirmative diagnosis of YGNNV infection.

Original language	English
Pages (from-to)	237-244
Number of pages	8
Journal	Journal of Fish Diseases
Volume	24
Issue number	4
DOIs	https://doi.org/10.1046/j.1365-2761.2001.00293.x
Publication status	Published - 2001

Keywords

Diagnosis
Epinephelus awoara
Immunohistochemistry
MAbs
Neutralization
YGNNV

ASJC Scopus subject areas

Aquatic Science
veterinary (miscalleneous)

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Lai, Y. S., Chiu, H. C., Murali, S., Guo, I. C., Chen, S. C., Fang, K., & Chang, C. Y. (2001). In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel). Journal of Fish Diseases, 24(4), 237-244. https://doi.org/10.1046/j.1365-2761.2001.00293.x

In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel). / Lai, Y. S.; Chiu, H. C.; Murali, S. et al.
In: Journal of Fish Diseases, Vol. 24, No. 4, 2001, p. 237-244.

Research output: Contribution to journal › Article › peer-review

Lai, YS, Chiu, HC, Murali, S, Guo, IC, Chen, SC, Fang, K & Chang, CY 2001, 'In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel)', Journal of Fish Diseases, vol. 24, no. 4, pp. 237-244. https://doi.org/10.1046/j.1365-2761.2001.00293.x

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abstract = "Mouse monoclonal antibodies (MAbs) were produced by using yellow grouper nervous necrosis virus (YGNNV) as an immunogen, isolated from infected yellow grouper, Epinephelus awoara (Temminck & Schlegel), and propagated in GB cells. In enzyme linked immunosorbent assay (ELISA), 43 hybridoma clones secreting MAbs strongly reacted with the purified virus. Ten of them showed a higher neutralization index (NI) value between 6.5 and 4.5 (log10 NI) than the other 33 MAbs against YGNNV infection in cell culture. All 10 MAbs belonged to the IgG isotype with a K light chain and recognized the 42 kDa coat protein of YGNNV by Western blot analysis. Immunohistochemical results demonstrated that the viral signals co-located with pathological lesions observed in retina, brain and spinal cord. These results indicate that the MAbs are useful for confirmative diagnosis of YGNNV infection.",

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AU - Lai, Y. S.

AU - Chiu, H. C.

AU - Murali, S.

AU - Guo, I. C.

AU - Chen, S. C.

AU - Fang, K.

AU - Chang, C. Y.

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N2 - Mouse monoclonal antibodies (MAbs) were produced by using yellow grouper nervous necrosis virus (YGNNV) as an immunogen, isolated from infected yellow grouper, Epinephelus awoara (Temminck & Schlegel), and propagated in GB cells. In enzyme linked immunosorbent assay (ELISA), 43 hybridoma clones secreting MAbs strongly reacted with the purified virus. Ten of them showed a higher neutralization index (NI) value between 6.5 and 4.5 (log10 NI) than the other 33 MAbs against YGNNV infection in cell culture. All 10 MAbs belonged to the IgG isotype with a K light chain and recognized the 42 kDa coat protein of YGNNV by Western blot analysis. Immunohistochemical results demonstrated that the viral signals co-located with pathological lesions observed in retina, brain and spinal cord. These results indicate that the MAbs are useful for confirmative diagnosis of YGNNV infection.

AB - Mouse monoclonal antibodies (MAbs) were produced by using yellow grouper nervous necrosis virus (YGNNV) as an immunogen, isolated from infected yellow grouper, Epinephelus awoara (Temminck & Schlegel), and propagated in GB cells. In enzyme linked immunosorbent assay (ELISA), 43 hybridoma clones secreting MAbs strongly reacted with the purified virus. Ten of them showed a higher neutralization index (NI) value between 6.5 and 4.5 (log10 NI) than the other 33 MAbs against YGNNV infection in cell culture. All 10 MAbs belonged to the IgG isotype with a K light chain and recognized the 42 kDa coat protein of YGNNV by Western blot analysis. Immunohistochemical results demonstrated that the viral signals co-located with pathological lesions observed in retina, brain and spinal cord. These results indicate that the MAbs are useful for confirmative diagnosis of YGNNV infection.

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In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel)

Abstract

Keywords

ASJC Scopus subject areas

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