In vitro efficiency of intra- and extracellular immunization with mouse anti-YGNNV antibody against yellow grouper nervous necrosis virus

Yu Shen Lai; Joseph Abraham Christopher John; Ing Cherng Guo; Shih Chieh Chen; Kang Fang; Chi Yao Chang

doi:10.1016/S0264-410X(02)00239-6

In vitro efficiency of intra- and extracellular immunization with mouse anti-YGNNV antibody against yellow grouper nervous necrosis virus

Yu Shen Lai, Joseph Abraham Christopher John, Ing Cherng Guo, Shih Chieh Chen, Kang Fang, Chi Yao Chang^*

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Mouse monoclonal antibody (MAb-18) against yellow grouper nervous necrosis virus (YGNNV) coat protein was developed and assayed for its neutralization ability. In the present study, we cloned and sequenced the cDNAs encoding heavy (γ) and light (κ) chains by constructing a cDNA library of the MAb-18 hybridoma. Three expression vectors, pCMV-NNV-18H (γ), pCMV-NNV-18L (κ), and pCMV-NNV-18HL (both γ and κ chains) were constructed and successfully expressed in grouper brain (GB) cells. Western blotting results indicated the secretion of antibody in to the medium with successful folding. Extracellular antibodies secreted by the pCMV-NNV-18HL transfected GB cells, neutralized well with YGNNV and showed the highest neutralization index (log₁₀ of NI) value 4. A significant reduction of titre (99.9%) was observed, when the intracellularly immunized GB cells were propagated with the YGNNV. These preliminary demonstrations suggest the immunoprophylactic use of plasmid constructs encoding the genes of mouse MAbs and the possibility of producing transgenic pathogen-free spawners and larvae, which contain freely available MAbs against pathogen in the circulation.

Original language	English
Pages (from-to)	3221-3229
Number of pages	9
Journal	Vaccine
Volume	20
Issue number	25-26
DOIs	https://doi.org/10.1016/S0264-410X(02)00239-6
Publication status	Published - 2002 Aug 19

Keywords

Fish
Immunoprophylaxis
Intrabody
Neutralization
VNN

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0264-410X(02)00239-6

Cite this

@article{167acbdea59f4c73ae7153762b34f68c,

title = "In vitro efficiency of intra- and extracellular immunization with mouse anti-YGNNV antibody against yellow grouper nervous necrosis virus",

abstract = "Mouse monoclonal antibody (MAb-18) against yellow grouper nervous necrosis virus (YGNNV) coat protein was developed and assayed for its neutralization ability. In the present study, we cloned and sequenced the cDNAs encoding heavy (γ) and light (κ) chains by constructing a cDNA library of the MAb-18 hybridoma. Three expression vectors, pCMV-NNV-18H (γ), pCMV-NNV-18L (κ), and pCMV-NNV-18HL (both γ and κ chains) were constructed and successfully expressed in grouper brain (GB) cells. Western blotting results indicated the secretion of antibody in to the medium with successful folding. Extracellular antibodies secreted by the pCMV-NNV-18HL transfected GB cells, neutralized well with YGNNV and showed the highest neutralization index (log10 of NI) value 4. A significant reduction of titre (99.9%) was observed, when the intracellularly immunized GB cells were propagated with the YGNNV. These preliminary demonstrations suggest the immunoprophylactic use of plasmid constructs encoding the genes of mouse MAbs and the possibility of producing transgenic pathogen-free spawners and larvae, which contain freely available MAbs against pathogen in the circulation.",

keywords = "Fish, Immunoprophylaxis, Intrabody, Neutralization, VNN",

author = "Lai, {Yu Shen} and John, {Joseph Abraham Christopher} and Guo, {Ing Cherng} and Chen, {Shih Chieh} and Kang Fang and Chang, {Chi Yao}",

note = "Funding Information: The authors wish to thank Dr. Steve R. Roffler, for the prediction of antibody domains. This work was supported by National Science Council, Taiwan, under the grant NSC 90-2311-B-001-129. ",

year = "2002",

month = aug,

day = "19",

doi = "10.1016/S0264-410X(02)00239-6",

language = "English",

volume = "20",

pages = "3221--3229",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "25-26",

}

TY - JOUR

T1 - In vitro efficiency of intra- and extracellular immunization with mouse anti-YGNNV antibody against yellow grouper nervous necrosis virus

AU - Lai, Yu Shen

AU - John, Joseph Abraham Christopher

AU - Guo, Ing Cherng

AU - Chen, Shih Chieh

AU - Fang, Kang

AU - Chang, Chi Yao

N1 - Funding Information: The authors wish to thank Dr. Steve R. Roffler, for the prediction of antibody domains. This work was supported by National Science Council, Taiwan, under the grant NSC 90-2311-B-001-129.

PY - 2002/8/19

Y1 - 2002/8/19

N2 - Mouse monoclonal antibody (MAb-18) against yellow grouper nervous necrosis virus (YGNNV) coat protein was developed and assayed for its neutralization ability. In the present study, we cloned and sequenced the cDNAs encoding heavy (γ) and light (κ) chains by constructing a cDNA library of the MAb-18 hybridoma. Three expression vectors, pCMV-NNV-18H (γ), pCMV-NNV-18L (κ), and pCMV-NNV-18HL (both γ and κ chains) were constructed and successfully expressed in grouper brain (GB) cells. Western blotting results indicated the secretion of antibody in to the medium with successful folding. Extracellular antibodies secreted by the pCMV-NNV-18HL transfected GB cells, neutralized well with YGNNV and showed the highest neutralization index (log10 of NI) value 4. A significant reduction of titre (99.9%) was observed, when the intracellularly immunized GB cells were propagated with the YGNNV. These preliminary demonstrations suggest the immunoprophylactic use of plasmid constructs encoding the genes of mouse MAbs and the possibility of producing transgenic pathogen-free spawners and larvae, which contain freely available MAbs against pathogen in the circulation.

AB - Mouse monoclonal antibody (MAb-18) against yellow grouper nervous necrosis virus (YGNNV) coat protein was developed and assayed for its neutralization ability. In the present study, we cloned and sequenced the cDNAs encoding heavy (γ) and light (κ) chains by constructing a cDNA library of the MAb-18 hybridoma. Three expression vectors, pCMV-NNV-18H (γ), pCMV-NNV-18L (κ), and pCMV-NNV-18HL (both γ and κ chains) were constructed and successfully expressed in grouper brain (GB) cells. Western blotting results indicated the secretion of antibody in to the medium with successful folding. Extracellular antibodies secreted by the pCMV-NNV-18HL transfected GB cells, neutralized well with YGNNV and showed the highest neutralization index (log10 of NI) value 4. A significant reduction of titre (99.9%) was observed, when the intracellularly immunized GB cells were propagated with the YGNNV. These preliminary demonstrations suggest the immunoprophylactic use of plasmid constructs encoding the genes of mouse MAbs and the possibility of producing transgenic pathogen-free spawners and larvae, which contain freely available MAbs against pathogen in the circulation.

KW - Fish

KW - Immunoprophylaxis

KW - Intrabody

KW - Neutralization

KW - VNN

UR - http://www.scopus.com/inward/record.url?scp=0037135682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037135682&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(02)00239-6

DO - 10.1016/S0264-410X(02)00239-6

M3 - Article

C2 - 12163274

AN - SCOPUS:0037135682

SN - 0264-410X

VL - 20

SP - 3221

EP - 3229

JO - Vaccine

JF - Vaccine

IS - 25-26

ER -

In vitro efficiency of intra- and extracellular immunization with mouse anti-YGNNV antibody against yellow grouper nervous necrosis virus

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this