TY - GEN
T1 - In-vitro diagnosis of colon cancer using bio-functionalized magnetic nanoparticles
AU - Hong, Chin Yih
AU - Yang, Shieh Yueh
AU - Huang, K. W.
AU - Horng, Herng Er
AU - Yang, Hong Chang
PY - 2011
Y1 - 2011
N2 - The popular bio-marker for colon cancer is carcinoembryonic antigen (CEA). By conjugating anti-CEA onto magnetic nanoparticles, CEA can be specially labeled and detected by measuring magnetic signals via immunomagnetic reduction (IMR). The low detection limit and detection range of IMR on CEA are investigated. The results are compared with those by using the existing assay, such as enzyme-linked immunosorbent assay (ELISA). It is evidenced that IMR has sensitivity much higher than that of ELISA. The low detection limit is below the normal level of CEA concentration of clinic practice and is suitable for early-stage in-vitro diagnosis for colon cancer. Furthermore, the dynamic range of detection for the CEA concentration using IMR extends well above the threshold of high risk level of colorectal carcinoma in current diagnosing practice. Therefore, IMR is also suitable in other stages diagnosis for colon cancer development.
AB - The popular bio-marker for colon cancer is carcinoembryonic antigen (CEA). By conjugating anti-CEA onto magnetic nanoparticles, CEA can be specially labeled and detected by measuring magnetic signals via immunomagnetic reduction (IMR). The low detection limit and detection range of IMR on CEA are investigated. The results are compared with those by using the existing assay, such as enzyme-linked immunosorbent assay (ELISA). It is evidenced that IMR has sensitivity much higher than that of ELISA. The low detection limit is below the normal level of CEA concentration of clinic practice and is suitable for early-stage in-vitro diagnosis for colon cancer. Furthermore, the dynamic range of detection for the CEA concentration using IMR extends well above the threshold of high risk level of colorectal carcinoma in current diagnosing practice. Therefore, IMR is also suitable in other stages diagnosis for colon cancer development.
KW - Carcinoembryonic antigen
KW - Colorectal carcinoma
KW - Enzyme-linked immunosorbent assay
KW - Immunomagnetic reduction
UR - http://www.scopus.com/inward/record.url?scp=84863591990&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863591990&partnerID=8YFLogxK
U2 - 10.1115/DETC2011-47178
DO - 10.1115/DETC2011-47178
M3 - Conference contribution
AN - SCOPUS:84863591990
SN - 9780791854846
T3 - Proceedings of the ASME Design Engineering Technical Conference
SP - 11
EP - 15
BT - ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE 2011
T2 - ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, IDETC/CIE 2011
Y2 - 28 August 2011 through 31 August 2011
ER -
