In vitro characterization and in vivo application of a dual functional peptide

Chien Jung Chen, Ping Hsueh Kuo, Ta Jen Hung, Shun Lung Fang, Che Chuan Yang, Shieh-Yueh Yang, Margaret Dah Tsyr Chang

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    Heparan sulfate (HS), one kind of sulfated glycosaminoglycans (GAGs), plays an important role in growth factor-receptor interaction, thereby involving in growth, angiogenesis and innate immune signaling. It is also highly addressed that GAGs and their anchoring core proteins are abnormally expressed in various cancers including lung, liver, and colon cancers. Human ribonuclease A (RNaseA) family members, hRNase2, hRNase3 and hRNase5 are reported to bind to cell surface by recognition of sulfated GAGs. We have recently identified three functionally important HS and heparin binding regions in hRNase3, also named as human eosinophil cationic protein (ECP), and characterized a dual-functional peptide (CPPecp) with GAG-binding and cell-penetrating activities. Screening of in vitro binding activity on various gastrointestinal cell lines demonstrated that CPPecp bound to cells rich in HS on the cell surface. However, cellular binding activity of FITC-CPPecp was abolished while cell surface HS was removed by haparinase treatment. In order to characterize the specific binding of CPPecp, quantitative assay was carried out to precisely decipher binding affinity between CPPecp and a variety of GAGs. In addition, magnetic nanoparticle (Fe3O4)-conjugated CPPecp (MNP-CPPecp) was synthesized as a novel bioprobe to evaluate its feasibility in in vivo magnetic resonance imaging (MRI) analysis in colon tumor mouse model. Taken together, our CPPecp in vitro preferred to bind to sulfated GAGs and selectively attach to HS on cell surface. Moreover, CPPecp demonstrated in vivo tumor targeting activity in colon tumor mouse model, suggesting that CPPecp possesses high potential to be developed as a novel molecular imaging agent and cancer targeting delivery vector.

    Original languageEnglish
    Title of host publicationProceedings - 2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013
    Pages576-581
    Number of pages6
    DOIs
    Publication statusPublished - 2013 Oct 15
    Event2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013 - Taichung, Taiwan
    Duration: 2013 Jul 32013 Jul 5

    Publication series

    NameProceedings - 2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013

    Other

    Other2013 7th International Conference on Complex, Intelligent, and Software Intensive Systems, CISIS 2013
    CountryTaiwan
    CityTaichung
    Period13/7/313/7/5

    ASJC Scopus subject areas

    • Artificial Intelligence
    • Software

    Fingerprint Dive into the research topics of 'In vitro characterization and in vivo application of a dual functional peptide'. Together they form a unique fingerprint.

    Cite this