Immunomagnetic reduction assay on chloramphenicol extracted from shrimp

S. Y. Yang; C. S. Ho; C. L. Lee; B. Y. Shih; H. E. Horng; Chin Yih Hong; H. C. Yang; Y. H. Chung; J. C. Chen; T. C. Lin

doi:10.1016/j.foodchem.2011.09.064

Immunomagnetic reduction assay on chloramphenicol extracted from shrimp

S. Y. Yang, C. S. Ho, C. L. Lee, B. Y. Shih, H. E. Horng^*, Chin Yih Hong, H. C. Yang, Y. H. Chung, J. C. Chen, T. C. Lin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The application of the assay methodology, called immunomagnetic reduction, using bio-functionalized magnetic nanoparticles as labeling markers for chloramphenicol was investigated. The reduction in the alternative-current (ac) magnetic susceptibility χ_ac of magnetic nanoparticles caused by the association between magnetic nanoparticles and chloramphenicol was detected as a function of the concentration of chloramphenicol. In this study, the characterizations used to detect chloramphenicol, such as low-detection limit and interference, were also conducted. Furthermore, the extracting processes for chloramphenicol from shrimp were explored. Thus, the platform for detecting chloramphenicol residue in shrimp via immunomagnetic reduction was demonstrated. Such platform showed features of a 0.1-ppb low-detection limit, low interference from other kinds of antibiotics, and easy operation.

Original language	English
Pages (from-to)	1021-1025
Number of pages	5
Journal	Food Chemistry
Volume	131
Issue number	3
DOIs	https://doi.org/10.1016/j.foodchem.2011.09.064
Publication status	Published - 2012 Apr 1
Externally published	Yes

Keywords

Chloramphenicol
Immunomagnetic reduction
Magnetic nanoparticles

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2011.09.064

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title = "Immunomagnetic reduction assay on chloramphenicol extracted from shrimp",

abstract = "The application of the assay methodology, called immunomagnetic reduction, using bio-functionalized magnetic nanoparticles as labeling markers for chloramphenicol was investigated. The reduction in the alternative-current (ac) magnetic susceptibility χac of magnetic nanoparticles caused by the association between magnetic nanoparticles and chloramphenicol was detected as a function of the concentration of chloramphenicol. In this study, the characterizations used to detect chloramphenicol, such as low-detection limit and interference, were also conducted. Furthermore, the extracting processes for chloramphenicol from shrimp were explored. Thus, the platform for detecting chloramphenicol residue in shrimp via immunomagnetic reduction was demonstrated. Such platform showed features of a 0.1-ppb low-detection limit, low interference from other kinds of antibiotics, and easy operation.",

keywords = "Chloramphenicol, Immunomagnetic reduction, Magnetic nanoparticles",

author = "Yang, {S. Y.} and Ho, {C. S.} and Lee, {C. L.} and Shih, {B. Y.} and Horng, {H. E.} and Hong, {Chin Yih} and Yang, {H. C.} and Chung, {Y. H.} and Chen, {J. C.} and Lin, {T. C.}",

note = "Funding Information: This work has been supported by the National Science Council of Taiwan under Grant Numbers 98-2112-M-003-003, 98-2323-B-003-001-CC2, and by the Department of Health under Grant Numbers DOH98-TD-N-111-008, and NSC98-2752-M-002-016-PAE, and by the Ministry of Economic Affairs of Taiwan under Grant Numbers 1Z970688 (SBIR), 1Z0990415 (SBIR), and S09800226-203 (JAID).",

year = "2012",

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doi = "10.1016/j.foodchem.2011.09.064",

language = "English",

volume = "131",

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journal = "Food Chemistry",

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T1 - Immunomagnetic reduction assay on chloramphenicol extracted from shrimp

AU - Yang, S. Y.

AU - Ho, C. S.

AU - Lee, C. L.

AU - Shih, B. Y.

AU - Horng, H. E.

AU - Hong, Chin Yih

AU - Yang, H. C.

AU - Chung, Y. H.

AU - Chen, J. C.

AU - Lin, T. C.

N1 - Funding Information: This work has been supported by the National Science Council of Taiwan under Grant Numbers 98-2112-M-003-003, 98-2323-B-003-001-CC2, and by the Department of Health under Grant Numbers DOH98-TD-N-111-008, and NSC98-2752-M-002-016-PAE, and by the Ministry of Economic Affairs of Taiwan under Grant Numbers 1Z970688 (SBIR), 1Z0990415 (SBIR), and S09800226-203 (JAID).

PY - 2012/4/1

Y1 - 2012/4/1

N2 - The application of the assay methodology, called immunomagnetic reduction, using bio-functionalized magnetic nanoparticles as labeling markers for chloramphenicol was investigated. The reduction in the alternative-current (ac) magnetic susceptibility χac of magnetic nanoparticles caused by the association between magnetic nanoparticles and chloramphenicol was detected as a function of the concentration of chloramphenicol. In this study, the characterizations used to detect chloramphenicol, such as low-detection limit and interference, were also conducted. Furthermore, the extracting processes for chloramphenicol from shrimp were explored. Thus, the platform for detecting chloramphenicol residue in shrimp via immunomagnetic reduction was demonstrated. Such platform showed features of a 0.1-ppb low-detection limit, low interference from other kinds of antibiotics, and easy operation.

AB - The application of the assay methodology, called immunomagnetic reduction, using bio-functionalized magnetic nanoparticles as labeling markers for chloramphenicol was investigated. The reduction in the alternative-current (ac) magnetic susceptibility χac of magnetic nanoparticles caused by the association between magnetic nanoparticles and chloramphenicol was detected as a function of the concentration of chloramphenicol. In this study, the characterizations used to detect chloramphenicol, such as low-detection limit and interference, were also conducted. Furthermore, the extracting processes for chloramphenicol from shrimp were explored. Thus, the platform for detecting chloramphenicol residue in shrimp via immunomagnetic reduction was demonstrated. Such platform showed features of a 0.1-ppb low-detection limit, low interference from other kinds of antibiotics, and easy operation.

KW - Chloramphenicol

KW - Immunomagnetic reduction

KW - Magnetic nanoparticles

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Immunomagnetic reduction assay on chloramphenicol extracted from shrimp

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Keywords

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