Identifying specific and differentially linked glycosyl residues in Mammalian glycans by targeted LC-MS analysis

Yi Li Huang, Bing Yi Jhou, Sung Fang Chen, Kay Hooi Khoo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glycans, which are widespread in nature, consist of a large number of monosaccharides linked via glycosidic bonds. Due to the complex nature of glycan structures on glycoproteins, assessing the configuration and positions of the glycosidic linkages of a glycan is a subject of considerable interest. In this study, a method for accomplishing this using partially O-methylated alditols (PMAs) from glycans combined with LC-MS analysis is reported. N-Glycans were first permethylated with methyl iodide, and the levels of methylation were further confirmed by MALDI-TOF. PMAs were then produced via complete hydrolysis and reduction. PMAs derived from Fetuin N-glycan and Lewis a antigen carbohydrates were successfully detected by LC-MS analysis. This analysis can be performed without the need for an additional derivatization step for GC analysis, and should be suitable for use in conjunction with a LC-MS-based analysis platform.

Original languageEnglish
Pages (from-to)1049-1054
Number of pages6
JournalAnalytical Sciences
Volume34
Issue number9
DOIs
Publication statusPublished - 2018 Jan 1

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Keywords

  • LC-MS
  • Linkage analysis
  • Partially methylated alditol

ASJC Scopus subject areas

  • Analytical Chemistry

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