Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis

Choun Sea Lin, Yi Hwa Lai, Chih Wen Sun, Nien Tzu Liu, Hsing Shen Tsay, Wei Chin Chang, Jeremy J.W. Chen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To gain a better understanding of gene expression in bamboo (Bambusa edulis Murno), we have used a combination of suppressive subtractive hybridization (SSH), microarray hybridization analysis, sequencing, and bioinformatics to identify bamboo genes differentially expressed in a bamboo albino mutant. Ten expressed sequence tags (ESTs) were found to be differentially expressed; these were isolated and sequenced. RT-PCR analysis of these ESTs supported the results of the microarray analysis. Six ESTs that were nucleus-encoded exhibited differential expression patterns in the green wild-type bamboo relative to the albino mutant. These genes (exception being the Rubisco small subunit) were non-photosynthesis-related genes. The development of a specific SSH cDNA library in which most of the chloroplast-encoded or photosynthesis-related genes had been subtracted proved to be useful for studying the function of non-photosynthesis-related genes in the albino bamboo mutants with aberrant chloroplast genome. The combined use of this SSH library with microarray analysis will provide a powerful analytical tool for future studies of the bamboo genome.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume86
Issue number2
DOIs
Publication statusPublished - 2006 Aug 1

ASJC Scopus subject areas

  • Horticulture

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