Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry

Li Chan Lin; Ju Tsung Liu; Shiu Huey Chou; Cheng Huang Lin

doi:10.1016/j.jchromb.2003.09.052

Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry

Li Chan Lin
, Ju Tsung Liu
, Shiu Huey Chou
, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (M_w: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (M_w: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (M_w: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M _w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

Original language	English
Pages (from-to)	241-247
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	798
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2003.09.052
Publication status	Published - 2003 Dec 25

Keywords

2,5-Dimethoxy-4-ethylthiophenethylamine

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/j.jchromb.2003.09.052

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Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry. / Lin, Li Chan; Liu, Ju Tsung; Chou, Shiu Huey et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 798, No. 2, 25.12.2003, p. 241-247.

Research output: Contribution to journal › Article › peer-review

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title = "Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry",

abstract = "A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.",

keywords = "2,5-Dimethoxy-4-ethylthiophenethylamine",

author = "Lin, \{Li Chan\} and Liu, \{Ju Tsung\} and Chou, \{Shiu Huey\} and Lin, \{Cheng Huang\}",

note = "Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-91-2113-M-003-016. The authors thank Prof. Ching-Fa Yao for the helpful discussions.",

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doi = "10.1016/j.jchromb.2003.09.052",

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AU - Chou, Shiu Huey

AU - Lin, Cheng Huang

N1 - Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-91-2113-M-003-016. The authors thank Prof. Ching-Fa Yao for the helpful discussions.

PY - 2003/12/25

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N2 - A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

AB - A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

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Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry

Abstract

Keywords

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