Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry

Li Chan Lin, Ju Tsung Liu, Shiu Huey Chou, Cheng-Huang Lin

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume798
Issue number2
DOIs
Publication statusPublished - 2003 Dec 25

Fingerprint

Metabolites
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Rats
Ethane
Urine
Ions
Liquid-Liquid Extraction
Monitoring
Liquids
Mass spectrometers
Metabolic Networks and Pathways
Metabolism
Acetic Acid
Gas Chromatography
Hydrolysis
Mass Spectrometry
Ethanol
Injections

Keywords

  • 2,5-Dimethoxy-4-ethylthiophenethylamine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

@article{b355f3458f80405ca7d4cfe7bb037ce8,
title = "Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry",
abstract = "A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.",
keywords = "2,5-Dimethoxy-4-ethylthiophenethylamine",
author = "Lin, {Li Chan} and Liu, {Ju Tsung} and Chou, {Shiu Huey} and Cheng-Huang Lin",
year = "2003",
month = "12",
day = "25",
doi = "10.1016/j.jchromb.2003.09.052",
language = "English",
volume = "798",
pages = "241--247",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry

AU - Lin, Li Chan

AU - Liu, Ju Tsung

AU - Chou, Shiu Huey

AU - Lin, Cheng-Huang

PY - 2003/12/25

Y1 - 2003/12/25

N2 - A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

AB - A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20mg/kg of 2C-T-2 by intra-peritoneal injection, and 24h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (M w: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.

KW - 2,5-Dimethoxy-4-ethylthiophenethylamine

UR - http://www.scopus.com/inward/record.url?scp=0345276753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345276753&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2003.09.052

DO - 10.1016/j.jchromb.2003.09.052

M3 - Article

C2 - 14643503

AN - SCOPUS:0345276753

VL - 798

SP - 241

EP - 247

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 2

ER -