Hypoxia preconditioning attenuates bladder overdistension-induced oxidative injury by up-regulation of Bcl-2 in the rat

Hong Jeng Yu, Chiang-Ting Chien, Yu Jen Lai, Ming Kuen Lai, Chau Fong Chen, Robert M. Levin, Su Ming Hsu

Research output: Contribution to journalArticle

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Abstract

We explored whether hypoxic preconditioning minimizes oxidative injury induced by overdistension/emptying in the rat bladder. For hypoxic preconditioning, female Wistar rats were placed in a hypobaric chamber (380 Torr) 15 h day-1 for 28 days. Overdistension was induced by infusion of two times the threshold volume of saline into the bladder and was maintained for 1 or 2 h, followed by drainage/emptying. During overdistension (ischaemia) and emptying (reperfusion) periods, a bursting increase of reactive oxygen species (ROS) from the bladder was originated from the large numbers of infiltrating leucocytes and scattered resident cells, including urothelial, submucosal, and smooth muscle cells. ROS impaired the voiding function by a reduction of bladder afferent and efferent nerve activity and bethanecol-or ATP-induced detrusor contraction. ROS enhanced pro-apoptotic mechanisms, including increases in the Bax/Bcl-2 ratio, CPP32 expression, and poly(ADP-ribose) polymerase (PARP) fragments with subsequent apoptotic cell formation in the insulted bladders. Hypoxia preconditioning up-regulated Bcl-2 expression in the bladder and significantly reduced the levels of ROS and apoptosis detected in the overdistension/emptying bladders and preserved partial voiding function. Bcl-2 up-regulation by hypoxia preconditioning contributes protection against overdistension/emptying-induced oxidative stress and injury in the bladder.

Original languageEnglish
Pages (from-to)815-828
Number of pages14
JournalJournal of Physiology
Volume554
Issue number3
DOIs
Publication statusPublished - 2004 Feb 1

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Urinary Bladder
Up-Regulation
Wounds and Injuries
Reactive Oxygen Species
Bethanechol
Poly(ADP-ribose) Polymerases
Hypoxia
Leukocyte Count
Reperfusion
Smooth Muscle Myocytes
Wistar Rats
Drainage
Oxidative Stress
Ischemia
Adenosine Triphosphate
Apoptosis

ASJC Scopus subject areas

  • Physiology

Cite this

Hypoxia preconditioning attenuates bladder overdistension-induced oxidative injury by up-regulation of Bcl-2 in the rat. / Yu, Hong Jeng; Chien, Chiang-Ting; Lai, Yu Jen; Lai, Ming Kuen; Chen, Chau Fong; Levin, Robert M.; Hsu, Su Ming.

In: Journal of Physiology, Vol. 554, No. 3, 01.02.2004, p. 815-828.

Research output: Contribution to journalArticle

Yu, Hong Jeng ; Chien, Chiang-Ting ; Lai, Yu Jen ; Lai, Ming Kuen ; Chen, Chau Fong ; Levin, Robert M. ; Hsu, Su Ming. / Hypoxia preconditioning attenuates bladder overdistension-induced oxidative injury by up-regulation of Bcl-2 in the rat. In: Journal of Physiology. 2004 ; Vol. 554, No. 3. pp. 815-828.
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