Hydrolysis catalyzed with a resin containing histidine groups

Wei Hsiu Hung, Cho Chun Hu, Chuen Ying Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A histidine-containing polymer was synthesized in which the amino group of the histidine was attached chemically via an azide coupling method to the carboxylic acid of Amberlite IRC-50. The resultant polymer was applied as a catalyst for hydrolysis of p-nitrophenyl acetate (PNPA). PNPA in aqueous solution was hydrolyzed at 25°C with a phosphate buffer (pH 7.8). The observed kinetics obeyed Michaelis-Menten kinetics. The reaction rates at various temperature were measured. The activation parameters, preexponential factor (A) and activation energy (Ea), were 6.64 × 10-4 min-1 and 37.5 kJ mol-1 respectively. At a pH of the medium greater than 7.8, the reaction rate remained almost constant (kobs = 0.024 min-1) and seemed to be controlled by the rate of diffusion of PNPA from the bulk solution into the catalytically active site at the resin channel surface. For catalysed hydrolysis, the effect of ionic strength in solution demonstrated that bifunctional cooperation between adjacent histidine groups existed through the nucleophilicity of nitrogen. The effects of metal ions and aspartic acid or serine on hydrolysis were also investigated.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalJournal of Molecular Catalysis A: Chemical
Volume106
Issue number1-2
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • Histidine-containing resin
  • Hydrolysis
  • p-Nitrophenyl acetate

ASJC Scopus subject areas

  • Catalysis
  • Process Chemistry and Technology
  • Physical and Theoretical Chemistry

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