Human apolipoprotein E: Correlation of polymorphisms and serum lipid concentrations in Chinese

Der Yan Tai, Fei Hung Su, Kuo Hsuan Chang, Guey Jen Lee-Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. The allelic variation of the apolipoprotein E (apoE) gene affects serum lipid concentrations. This study investigates the correlation of apoE polymorphisms and serum cholesterol and triglyceride concentrations in Chinese subjects. Methods. DNA from a random sample of 136 unrelated individuals was used for polymerase chain reaction (PCR) amplification of the three apoE alleles. The amplified products were analyzed by HhaI restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP). Results. Identical genotypes were obtained by both methods in all individuals analyzed. The three apoE alleles examined were in Hardy-Weinberg equilibrium (p = 0.417). The allele frequencies in individuals with cholesterol and triglyceride values of below 200 mg/dl were 8.6% for ε 2, 82.8% for ε 3 and 8.6% for ε 4. When allele frequency between individuals with cholesterol values of below and above 200 mg/dl was compared, there appeared little difference in the ε 2 and ε 4 alleles between the two groups (p = 0.823). Conversely, a statistically significant difference in the ε 2 allele was observed (p = 0.048) between individuals with triglyceride values of below and above 200 mg/dl. Conclusions. The results demonstrate that neither the ε 2 nor ε 4 alleles affect cholesterol values in Chinese subjects, whereas the ε 2 allele exerts an influence on serum triglyceride concentrations in Chinese.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalChinese Medical Journal (Taipei)
Volume62
Issue number3
Publication statusPublished - 1999 Mar

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Apolipoproteins E
Alleles
Lipids
Triglycerides
Serum
Cholesterol
Gene Frequency
Restriction Fragment Length Polymorphisms
Genotype
Polymerase Chain Reaction
DNA
Genes

Keywords

  • Apolipoprotein E
  • Cholesterol
  • Polymorphism
  • Triglyceride

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Human apolipoprotein E : Correlation of polymorphisms and serum lipid concentrations in Chinese. / Tai, Der Yan; Su, Fei Hung; Chang, Kuo Hsuan; Lee-Chen, Guey Jen.

In: Chinese Medical Journal (Taipei), Vol. 62, No. 3, 03.1999, p. 133-139.

Research output: Contribution to journalArticle

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abstract = "Background. The allelic variation of the apolipoprotein E (apoE) gene affects serum lipid concentrations. This study investigates the correlation of apoE polymorphisms and serum cholesterol and triglyceride concentrations in Chinese subjects. Methods. DNA from a random sample of 136 unrelated individuals was used for polymerase chain reaction (PCR) amplification of the three apoE alleles. The amplified products were analyzed by HhaI restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP). Results. Identical genotypes were obtained by both methods in all individuals analyzed. The three apoE alleles examined were in Hardy-Weinberg equilibrium (p = 0.417). The allele frequencies in individuals with cholesterol and triglyceride values of below 200 mg/dl were 8.6{\%} for ε 2, 82.8{\%} for ε 3 and 8.6{\%} for ε 4. When allele frequency between individuals with cholesterol values of below and above 200 mg/dl was compared, there appeared little difference in the ε 2 and ε 4 alleles between the two groups (p = 0.823). Conversely, a statistically significant difference in the ε 2 allele was observed (p = 0.048) between individuals with triglyceride values of below and above 200 mg/dl. Conclusions. The results demonstrate that neither the ε 2 nor ε 4 alleles affect cholesterol values in Chinese subjects, whereas the ε 2 allele exerts an influence on serum triglyceride concentrations in Chinese.",
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N2 - Background. The allelic variation of the apolipoprotein E (apoE) gene affects serum lipid concentrations. This study investigates the correlation of apoE polymorphisms and serum cholesterol and triglyceride concentrations in Chinese subjects. Methods. DNA from a random sample of 136 unrelated individuals was used for polymerase chain reaction (PCR) amplification of the three apoE alleles. The amplified products were analyzed by HhaI restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP). Results. Identical genotypes were obtained by both methods in all individuals analyzed. The three apoE alleles examined were in Hardy-Weinberg equilibrium (p = 0.417). The allele frequencies in individuals with cholesterol and triglyceride values of below 200 mg/dl were 8.6% for ε 2, 82.8% for ε 3 and 8.6% for ε 4. When allele frequency between individuals with cholesterol values of below and above 200 mg/dl was compared, there appeared little difference in the ε 2 and ε 4 alleles between the two groups (p = 0.823). Conversely, a statistically significant difference in the ε 2 allele was observed (p = 0.048) between individuals with triglyceride values of below and above 200 mg/dl. Conclusions. The results demonstrate that neither the ε 2 nor ε 4 alleles affect cholesterol values in Chinese subjects, whereas the ε 2 allele exerts an influence on serum triglyceride concentrations in Chinese.

AB - Background. The allelic variation of the apolipoprotein E (apoE) gene affects serum lipid concentrations. This study investigates the correlation of apoE polymorphisms and serum cholesterol and triglyceride concentrations in Chinese subjects. Methods. DNA from a random sample of 136 unrelated individuals was used for polymerase chain reaction (PCR) amplification of the three apoE alleles. The amplified products were analyzed by HhaI restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP). Results. Identical genotypes were obtained by both methods in all individuals analyzed. The three apoE alleles examined were in Hardy-Weinberg equilibrium (p = 0.417). The allele frequencies in individuals with cholesterol and triglyceride values of below 200 mg/dl were 8.6% for ε 2, 82.8% for ε 3 and 8.6% for ε 4. When allele frequency between individuals with cholesterol values of below and above 200 mg/dl was compared, there appeared little difference in the ε 2 and ε 4 alleles between the two groups (p = 0.823). Conversely, a statistically significant difference in the ε 2 allele was observed (p = 0.048) between individuals with triglyceride values of below and above 200 mg/dl. Conclusions. The results demonstrate that neither the ε 2 nor ε 4 alleles affect cholesterol values in Chinese subjects, whereas the ε 2 allele exerts an influence on serum triglyceride concentrations in Chinese.

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