Human α-L-iduronidase (IDUA) gene: Apparent recombination in intron 2 by haplotype analysis in a Taiwanese population

Guey Jen Lee-Chen, Tso Ren Wang, Wuh Liang Hwu, Kuen Rong Day, Cheng Kuang Wang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The polymorphic DNA haplotype of the α-L-iduronidase (IDUA) gene in a Taiwanese population was investigated. Genomic DNA extracted from 85 volunteers was used to amplify fragments containing the polymorphic sites A8, A20 and Q33H from exon 1 and a variable number of tandem repeats (VNTR) region in intron 2. Additionally, sites R105Q and L118 in exon 3, A314 from exon 7, A361T and T388 from exon 8, T410 and V454I from exon 9, and R489 from exon 10 were amplified. The polymerase chain reaction-amplified products were analyzed by restriction fragment length polymorphism (RFLP) analysis, allele specific oligonucleotide (ASO) hybridization, or gel electrophoresis. Of the examined polymorphisms, the intron 2 VNTR was not in Hardy-Weinberg equilibrium. Conversely, all 11 single base change polymorphisms were in Hardy-Weinberg equilibrium. Linkage disequilibrium analysis of the haplotype data revealed strong nonrandom association between A8 and A20 in exon 1 as well as among R105Q A314, A361T, T388, T410, V454I, and R489 in exons 3 to 10. In contrast, little linkage disequilibrium between two clusters of linked polymorphisms on either side of the VNTR was observed. The results suggest apparent recombination in intron 2 of the IDUA gene, with little or no recombination in exon 1 or exons 3 to 10.

Original languageEnglish
Pages (from-to)465-470
Number of pages6
JournalJournal of the Formosan Medical Association
Volume97
Issue number7
Publication statusPublished - 1998 Jul

Keywords

  • α-L-iduronidase polymorphism
  • Haplotype
  • Linkage disequilibrium

ASJC Scopus subject areas

  • Medicine(all)

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