Abstract
Hoxa 11 is a murine Abdominal-B-type homeobox gene. The structure of this gene is presented, including genomic and cDNA sequence. The cDNA includes the complete open reading frame and based on primer extension results is near full length. Surprisingly, the antisense strand of Hoxa 11 was found to be transcribed. Moreover, these antisense transcripts were processed and polyadenylated. The developmental expression patterns for both sense and antisense transcripts were examined using serial section and whole-mount in situ hybridizations. Hoxa 11 transcription patterns were defined in the limbs, kidney and stromal cells surrounding the Mullerian and Wolffian ducts. Of particular interest, in the developing limbs, the sense and antisense transcripts showed complementary expression patterns, with antisense RNAs increasing in abundance in regions where sense RNAs were diminishing in abundance. Furthermore, targeted mutation of Hoxa 11 is shown to result in both male and female sterility. The female mutants produce normal ova, which develop properly post-fertilization when transferred to wild-type surrogate mothers. The Hoxa 11 homozygous mutants are shown to provide a defective uterine environment. The mutant males exhibited a malformation of the vas deferens that resembles a partial homeotic transformation to an epididymis. In addition, the mutant testes fail to descend properly into the scrotum and, likely as a result, spermatogenesis is perturbed.
| Original language | English |
|---|---|
| Pages (from-to) | 1373-1385 |
| Number of pages | 13 |
| Journal | Development |
| Volume | 121 |
| Issue number | 5 |
| Publication status | Published - 1995 Jan 1 |
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Keywords
- Antisense transcripts
- Homeobox gene
- Limb development
- Mouse
- Spermatogenesis
- Uterus
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
Cite this
Hoxa 11 structure, extensive antisense transcription, and function in male and female fertility. / Hsieh-Li, H. M.; Witte, D. P.; Weinstein, M.; Branford, W.; Li, H.; Small, K.; Potter, S. S.
In: Development, Vol. 121, No. 5, 01.01.1995, p. 1373-1385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hoxa 11 structure, extensive antisense transcription, and function in male and female fertility
AU - Hsieh-Li, H. M.
AU - Witte, D. P.
AU - Weinstein, M.
AU - Branford, W.
AU - Li, H.
AU - Small, K.
AU - Potter, S. S.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Hoxa 11 is a murine Abdominal-B-type homeobox gene. The structure of this gene is presented, including genomic and cDNA sequence. The cDNA includes the complete open reading frame and based on primer extension results is near full length. Surprisingly, the antisense strand of Hoxa 11 was found to be transcribed. Moreover, these antisense transcripts were processed and polyadenylated. The developmental expression patterns for both sense and antisense transcripts were examined using serial section and whole-mount in situ hybridizations. Hoxa 11 transcription patterns were defined in the limbs, kidney and stromal cells surrounding the Mullerian and Wolffian ducts. Of particular interest, in the developing limbs, the sense and antisense transcripts showed complementary expression patterns, with antisense RNAs increasing in abundance in regions where sense RNAs were diminishing in abundance. Furthermore, targeted mutation of Hoxa 11 is shown to result in both male and female sterility. The female mutants produce normal ova, which develop properly post-fertilization when transferred to wild-type surrogate mothers. The Hoxa 11 homozygous mutants are shown to provide a defective uterine environment. The mutant males exhibited a malformation of the vas deferens that resembles a partial homeotic transformation to an epididymis. In addition, the mutant testes fail to descend properly into the scrotum and, likely as a result, spermatogenesis is perturbed.
AB - Hoxa 11 is a murine Abdominal-B-type homeobox gene. The structure of this gene is presented, including genomic and cDNA sequence. The cDNA includes the complete open reading frame and based on primer extension results is near full length. Surprisingly, the antisense strand of Hoxa 11 was found to be transcribed. Moreover, these antisense transcripts were processed and polyadenylated. The developmental expression patterns for both sense and antisense transcripts were examined using serial section and whole-mount in situ hybridizations. Hoxa 11 transcription patterns were defined in the limbs, kidney and stromal cells surrounding the Mullerian and Wolffian ducts. Of particular interest, in the developing limbs, the sense and antisense transcripts showed complementary expression patterns, with antisense RNAs increasing in abundance in regions where sense RNAs were diminishing in abundance. Furthermore, targeted mutation of Hoxa 11 is shown to result in both male and female sterility. The female mutants produce normal ova, which develop properly post-fertilization when transferred to wild-type surrogate mothers. The Hoxa 11 homozygous mutants are shown to provide a defective uterine environment. The mutant males exhibited a malformation of the vas deferens that resembles a partial homeotic transformation to an epididymis. In addition, the mutant testes fail to descend properly into the scrotum and, likely as a result, spermatogenesis is perturbed.
KW - Antisense transcripts
KW - Homeobox gene
KW - Limb development
KW - Mouse
KW - Spermatogenesis
KW - Uterus
UR - http://www.scopus.com/inward/record.url?scp=0029069318&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029069318&partnerID=8YFLogxK
M3 - Article
C2 - 7789268
AN - SCOPUS:0029069318
VL - 121
SP - 1373
EP - 1385
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 5
ER -