Holographic tomography: Techniques and biomedical applications [Invited]

Vinoth Balasubramani; Arkadiusz Kus; Han Yen Tu; Chau Jern Cheng; Maria Baczewska; Wojciech Krauze; Malgorzata Kujawinska

doi:10.1364/AO.416902

Holographic tomography: Techniques and biomedical applications [Invited]

Vinoth Balasubramani, Arkadiusz Kus, Han Yen Tu, Chau Jern Cheng, Maria Baczewska, Wojciech Krauze, Malgorzata Kujawinska^*

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Review article › peer-review

80 Citations (Scopus)

Abstract

Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies.HTuses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology.We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.

Original language	English
Pages (from-to)	B65-B80
Journal	Applied Optics
Volume	60
Issue number	10
DOIs	https://doi.org/10.1364/AO.416902
Publication status	Published - 2021 Apr 1

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

Access to Document

10.1364/AO.416902

Cite this

@article{bdb67597febf4c7f8642a763af17270a,

title = "Holographic tomography: Techniques and biomedical applications [Invited]",

abstract = "Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies.HTuses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology.We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.",

author = "Vinoth Balasubramani and Arkadiusz Kus and Tu, {Han Yen} and Cheng, {Chau Jern} and Maria Baczewska and Wojciech Krauze and Malgorzata Kujawinska",

note = "Publisher Copyright: {\textcopyright} 2021 Optical Society of America.",

year = "2021",

month = apr,

day = "1",

doi = "10.1364/AO.416902",

language = "English",

volume = "60",

pages = "B65--B80",

journal = "Applied Optics",

issn = "1559-128X",

publisher = "The Optical Society",

number = "10",

}

TY - JOUR

T1 - Holographic tomography

T2 - Techniques and biomedical applications [Invited]

AU - Balasubramani, Vinoth

AU - Kus, Arkadiusz

AU - Tu, Han Yen

AU - Cheng, Chau Jern

AU - Baczewska, Maria

AU - Krauze, Wojciech

AU - Kujawinska, Malgorzata

PY - 2021/4/1

Y1 - 2021/4/1

N2 - Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies.HTuses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology.We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.

AB - Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies.HTuses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology.We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.

UR - http://www.scopus.com/inward/record.url?scp=85103919481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85103919481&partnerID=8YFLogxK

U2 - 10.1364/AO.416902

DO - 10.1364/AO.416902

M3 - Review article

C2 - 33798138

AN - SCOPUS:85103919481

SN - 1559-128X

VL - 60

SP - B65-B80

JO - Applied Optics

JF - Applied Optics

IS - 10

ER -

Holographic tomography: Techniques and biomedical applications [Invited]

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this