Holographic tomography: Techniques and biomedical applications [Invited]

Vinoth Balasubramani, Arkadiusz Kus, Han Yen Tu, Chau Jern Cheng, Maria Baczewska, Wojciech Krauze, Malgorzata Kujawinska*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

69 Citations (Scopus)


Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies.HTuses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology.We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.

Original languageEnglish
Pages (from-to)B65-B80
JournalApplied Optics
Issue number10
Publication statusPublished - 2021 Apr 1

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering


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