Holographic Optical Tweezers: Techniques and Biomedical Applications

Hui Chi Chen*, Chau Jern Cheng

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)


Holographic optical tweezers (HOT) is a programmable technique used for manipulation of microsized samples. In combination with computer-generation holography (CGH), a spatial light modulator reshapes the light distribution within the focal area of the optical tweezers. HOT can be used to realize real-time multiple-point manipulation in fluid, and this is useful in biological research. In this article, we summarize the HOT technique, discuss its recent developments, and present an overview of its biological applications.

Original languageEnglish
Article number10244
JournalApplied Sciences (Switzerland)
Issue number20
Publication statusPublished - 2022 Oct


  • computer-generated hologram
  • holographic optical tweezers
  • holographic tomography
  • optical tweezers

ASJC Scopus subject areas

  • General Materials Science
  • Instrumentation
  • General Engineering
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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