TY - JOUR
T1 - Highly sensitive carboxyl-graphene oxide-based surface plasmon resonance immunosensor for the detection of lung cancer for cytokeratin 19 biomarker in human plasma
AU - Chiu, Nan Fu
AU - Lin, Ting Li
AU - Kuo, Chia Tzu
N1 - Funding Information:
The authors would like to thank the Ministry of Science and Technology of the Republic of China, Taiwan , for financially supporting this research under Contract No. MOST 103-2221-E-003-008 , MOST 104-2314-B-195-015 and MOST 105-2221-E-003-027 . We thank Dr. Yao-Jane Hsu groups for they help in analyzing XPS spectra (National Synchrotron Radiation Research Center, Beamline 09A2).
Funding Information:
The authors would like to thank the Ministry of Science and Technology of the Republic of China, Taiwan, for financially supporting this research under Contract No. MOST 103-2221-E-003-008, MOST 104-2314-B-195-015 and MOST 105-2221-E-003-027. We thank Dr. Yao-Jane Hsu groups for they help in analyzing XPS spectra (National Synchrotron Radiation Research Center, Beamline 09A2).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/7/15
Y1 - 2018/7/15
N2 - Functionalized graphene oxide is a novel type of highly efficient biosensing material. In this study, we present a carboxyl-functionalized graphene oxide (GO-COOH)-based surface plasmon resonance (SPR) chip for the rapid and quantitative detection of non-small cell lung carcinoma (NSCLC) via the cytolerayin 19 (CK19) protein biomarker in spiked human plasma. We demonstrated the binding specificity of kinetic analysis of interactions between GO-COOH and anti-CK19 and CK19 protein. We also calculated the relationship between the SPR angle and refractive index of GO-COOH, and demonstrated that –COOH modified GO sheets on Au film can enhance the field energy propagation intensity of an SPR sensor, resulting in a higher sensitivity for the detection of CK19 protein compared to a conventional Au-based SPR chip. The immunosensor was constructed and engineered by immobilizing a low concentration (10 μg/mL) of CK19 antibody on an SPR chip. The lowest detectable concentration was as low as 1 fg/mL. A spiked 10% human plasma CK19 detection limit of 0.05 pg/mL was achieved, well below the normal physiological level of serum protein (3.3 ng/mL). Therefore, a carboxyl-GO based SPR biosensor appears to have high sensitivity and specificity for the detection of clinical whole plasma biomarkers and possible application in diagnosing diseases.
AB - Functionalized graphene oxide is a novel type of highly efficient biosensing material. In this study, we present a carboxyl-functionalized graphene oxide (GO-COOH)-based surface plasmon resonance (SPR) chip for the rapid and quantitative detection of non-small cell lung carcinoma (NSCLC) via the cytolerayin 19 (CK19) protein biomarker in spiked human plasma. We demonstrated the binding specificity of kinetic analysis of interactions between GO-COOH and anti-CK19 and CK19 protein. We also calculated the relationship between the SPR angle and refractive index of GO-COOH, and demonstrated that –COOH modified GO sheets on Au film can enhance the field energy propagation intensity of an SPR sensor, resulting in a higher sensitivity for the detection of CK19 protein compared to a conventional Au-based SPR chip. The immunosensor was constructed and engineered by immobilizing a low concentration (10 μg/mL) of CK19 antibody on an SPR chip. The lowest detectable concentration was as low as 1 fg/mL. A spiked 10% human plasma CK19 detection limit of 0.05 pg/mL was achieved, well below the normal physiological level of serum protein (3.3 ng/mL). Therefore, a carboxyl-GO based SPR biosensor appears to have high sensitivity and specificity for the detection of clinical whole plasma biomarkers and possible application in diagnosing diseases.
KW - Carboxyl-functionalized graphene oxide (GO-COOH)
KW - Cytolerayin19 (CK19)
KW - Non-small cell lung carcinoma (NSCLC)
KW - Surface plasmon resonance (SPR)
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U2 - 10.1016/j.snb.2018.03.070
DO - 10.1016/j.snb.2018.03.070
M3 - Article
AN - SCOPUS:85044158916
SN - 0925-4005
VL - 265
SP - 264
EP - 272
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -
