Highly sensitive carboxyl-graphene oxide-based surface plasmon resonance immunosensor for the detection of lung cancer for cytokeratin 19 biomarker in human plasma

Nan Fu Chiu, Ting Li Lin, Chia Tzu Kuo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Functionalized graphene oxide is a novel type of highly efficient biosensing material. In this study, we present a carboxyl-functionalized graphene oxide (GO-COOH)-based surface plasmon resonance (SPR) chip for the rapid and quantitative detection of non-small cell lung carcinoma (NSCLC) via the cytolerayin 19 (CK19) protein biomarker in spiked human plasma. We demonstrated the binding specificity of kinetic analysis of interactions between GO-COOH and anti-CK19 and CK19 protein. We also calculated the relationship between the SPR angle and refractive index of GO-COOH, and demonstrated that –COOH modified GO sheets on Au film can enhance the field energy propagation intensity of an SPR sensor, resulting in a higher sensitivity for the detection of CK19 protein compared to a conventional Au-based SPR chip. The immunosensor was constructed and engineered by immobilizing a low concentration (10 μg/mL) of CK19 antibody on an SPR chip. The lowest detectable concentration was as low as 1 fg/mL. A spiked 10% human plasma CK19 detection limit of 0.05 pg/mL was achieved, well below the normal physiological level of serum protein (3.3 ng/mL). Therefore, a carboxyl-GO based SPR biosensor appears to have high sensitivity and specificity for the detection of clinical whole plasma biomarkers and possible application in diagnosing diseases.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume265
DOIs
Publication statusPublished - 2018 Jul 15

Fingerprint

Keratin-19
Plasma (human)
Immunosensors
Graphite
biomarkers
Surface plasmon resonance
Biomarkers
surface plasmon resonance
lungs
Oxides
Graphene
graphene
cancer
oxides
proteins
Proteins
chips
low concentrations
sensitivity
antibodies

Keywords

  • Carboxyl-functionalized graphene oxide (GO-COOH)
  • Cytolerayin19 (CK19)
  • Non-small cell lung carcinoma (NSCLC)
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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title = "Highly sensitive carboxyl-graphene oxide-based surface plasmon resonance immunosensor for the detection of lung cancer for cytokeratin 19 biomarker in human plasma",
abstract = "Functionalized graphene oxide is a novel type of highly efficient biosensing material. In this study, we present a carboxyl-functionalized graphene oxide (GO-COOH)-based surface plasmon resonance (SPR) chip for the rapid and quantitative detection of non-small cell lung carcinoma (NSCLC) via the cytolerayin 19 (CK19) protein biomarker in spiked human plasma. We demonstrated the binding specificity of kinetic analysis of interactions between GO-COOH and anti-CK19 and CK19 protein. We also calculated the relationship between the SPR angle and refractive index of GO-COOH, and demonstrated that –COOH modified GO sheets on Au film can enhance the field energy propagation intensity of an SPR sensor, resulting in a higher sensitivity for the detection of CK19 protein compared to a conventional Au-based SPR chip. The immunosensor was constructed and engineered by immobilizing a low concentration (10 μg/mL) of CK19 antibody on an SPR chip. The lowest detectable concentration was as low as 1 fg/mL. A spiked 10{\%} human plasma CK19 detection limit of 0.05 pg/mL was achieved, well below the normal physiological level of serum protein (3.3 ng/mL). Therefore, a carboxyl-GO based SPR biosensor appears to have high sensitivity and specificity for the detection of clinical whole plasma biomarkers and possible application in diagnosing diseases.",
keywords = "Carboxyl-functionalized graphene oxide (GO-COOH), Cytolerayin19 (CK19), Non-small cell lung carcinoma (NSCLC), Surface plasmon resonance (SPR)",
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AU - Chiu, Nan Fu

AU - Lin, Ting Li

AU - Kuo, Chia Tzu

PY - 2018/7/15

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N2 - Functionalized graphene oxide is a novel type of highly efficient biosensing material. In this study, we present a carboxyl-functionalized graphene oxide (GO-COOH)-based surface plasmon resonance (SPR) chip for the rapid and quantitative detection of non-small cell lung carcinoma (NSCLC) via the cytolerayin 19 (CK19) protein biomarker in spiked human plasma. We demonstrated the binding specificity of kinetic analysis of interactions between GO-COOH and anti-CK19 and CK19 protein. We also calculated the relationship between the SPR angle and refractive index of GO-COOH, and demonstrated that –COOH modified GO sheets on Au film can enhance the field energy propagation intensity of an SPR sensor, resulting in a higher sensitivity for the detection of CK19 protein compared to a conventional Au-based SPR chip. The immunosensor was constructed and engineered by immobilizing a low concentration (10 μg/mL) of CK19 antibody on an SPR chip. The lowest detectable concentration was as low as 1 fg/mL. A spiked 10% human plasma CK19 detection limit of 0.05 pg/mL was achieved, well below the normal physiological level of serum protein (3.3 ng/mL). Therefore, a carboxyl-GO based SPR biosensor appears to have high sensitivity and specificity for the detection of clinical whole plasma biomarkers and possible application in diagnosing diseases.

AB - Functionalized graphene oxide is a novel type of highly efficient biosensing material. In this study, we present a carboxyl-functionalized graphene oxide (GO-COOH)-based surface plasmon resonance (SPR) chip for the rapid and quantitative detection of non-small cell lung carcinoma (NSCLC) via the cytolerayin 19 (CK19) protein biomarker in spiked human plasma. We demonstrated the binding specificity of kinetic analysis of interactions between GO-COOH and anti-CK19 and CK19 protein. We also calculated the relationship between the SPR angle and refractive index of GO-COOH, and demonstrated that –COOH modified GO sheets on Au film can enhance the field energy propagation intensity of an SPR sensor, resulting in a higher sensitivity for the detection of CK19 protein compared to a conventional Au-based SPR chip. The immunosensor was constructed and engineered by immobilizing a low concentration (10 μg/mL) of CK19 antibody on an SPR chip. The lowest detectable concentration was as low as 1 fg/mL. A spiked 10% human plasma CK19 detection limit of 0.05 pg/mL was achieved, well below the normal physiological level of serum protein (3.3 ng/mL). Therefore, a carboxyl-GO based SPR biosensor appears to have high sensitivity and specificity for the detection of clinical whole plasma biomarkers and possible application in diagnosing diseases.

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